Production of isoquinoline alkaloids from the in vitro conserved Fumaria parviflora and their in vitro wound healing activity

Abstract

Fumaria parviflora Lam. is a rare herbaceous annual plant, with a well-known richness of isoquinoline alkaloids. It is threaten due to expansion on construction in the Mediterranean coastal region. We established callus culture protocol aiming at in vitro conservation of this plant. Murashige and Skoog medium fortified with a combination of 0.5 mg/l 1-naphthaleneacetic acid (NAA) and 1 mg/l 6-benzylaminopurine (BAP) showed optimal callus initiation. UPLC-MS/MS profiling revealed that calli induced on the tested media were able to produce isoquinoline alkaloids. Eight alkaloids were isolated from aerial parts of the cultivated plant and their cytotoxicity against Human skin fibroblast (HF) and wound healing activity using in vitro scratch assay were determined. Structural similarity between the isolated alkaloids enabled structure activity relationship (SAR) study. Sanguinarine displayed the potent activity compared to the other alkaloids. Iminium ion and methylenedioxy potentiated the activity.

Graphical Abstract

Keywords:

• Fumaria parviflora
• isoquinoline alkaloids
• callus culture
• cytotoxicity
• scratch assay

Disclosure statement

No potential conflict of interest was reported by the authors.