Elicitor mediated enhancement of shoot biomass and lupeol production in Hemidesmus indicus (L.) R. Br. ex. Schult. and Tylophora indica (Burm. F.) Merrill using yeast extract and salicylic acid

Abstract

Hemidesmus indicus (L.) R. Br. ex Schult. and Tylophora indica (Burm. F.) Merrill shoot cultures were treated with different concentrations of yeast extract (YE; 25–200 mg/L) and salicylic acid (SA; 50–200 µM), and their effect on lupeol production was assessed. The maximum dry weight (DW) biomass was recorded when H. indicus shoots were treated with SA (50 µM) and T. indica shoots with YE (200 mg/L). Highest lupeol yield (335.40 ± 0.04 µg/g DW) was obtained in H. indicus shoots after treatment with 50 µM of SA for 3 weeks. Whereas in T. indica, maximum lupeol content (584.26 ± 8.14 µg/g DW) was recorded by giving treatment with 25 μM of SA for 6 weeks.

Graphical Abstract

Keywords:

• Biomass
• elicitation
• HPTLC
• in vitro cultures
• lupeol
• medicinal plants

Acknowledgements

ARP, SRP and AGJ would like to thank University Grants Commission (UGC), New Delhi, India for providing DRS program to the Department. Director of PERD centre and Head of Department of Zoology, Biomedical Technology, Human Genetics, and Wildlife Biology & Conservation, University School of Sciences, Gujarat University are also acknowledged for providing HPTLC facilities.

Disclosure statement

All authors declare no conflict of interest.

Additional information

Funding

This work was supported by the University Grants Commission (UGC), New Delhi, India through BSR fellowship to ARP [F.No.7-319/2011(BSR)].