Spermacosides a and B, two new triterpenoid saponins from Spermacoce ocymoides burm.f.

Abstract

Two new triterpenoid saponins, named spermacosides A–B (1 − 2), together with two known oleanane-type triterpenoid saponins, 3-O-β-D-xylopyranosyl-(1→3)-β-D-glucopyranosylbayogenin (3) and 3-O-β-D-glucopyranosylbayogenin (4), were isolated from the ethyl acetate extract of Spermacoce ocymoides Burm.f. in a phytochemical investigation. Their chemical structures were elucidated by spectroscopic data analysis (1D and 2D NMR, and HR-ESI-MS), as well as comparison with reported data. All these compounds were evaluated for inhibiting nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 cells. Among them, 1 showed a slight effect with an IC₅₀ value of 108.65 ± 7.91 µM, and compounds 2–4 were inactive.

Graphical Abstract

Keywords:

• Rubiaceae
• spermacoce ocymoides
• borreria ocymoides
• triterpenoid saponins
• NO inhibitory activity

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Ho Chi Minh City University of Education Foundation for Science and Technology under grant number CS.2022.19.36.