Figure 1. Overview of the comparative secretomics workflow. Any kind of cell culture model can be analyzed. Therefore, the supernatant (secretome) and cells (intracellular proteome) are collected and prepared according to the media conditions. After proteolytic digestion, the peptides are analyzed by LC-MS/MS. The quantitative analysis will give access to bona fide secreted proteins and the qualitative peptide matching allows to draw conclusion about ectodomain shedding
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