Potential of vetiver (Vetiveria zizanioides (L.) Nash) for phytoremediation of petroleum hydrocarbon-contaminated soils in Venezuela

Abstract

Venezuela is one of the largest oil producers in the world. For the rehabilitation of oil-contaminated sites, phytoremediation represents a promising technology whereby plants are used to enhance biodegradation processes in soil. A greenhouse study was conducted to determine the tolerance of vetiver (Vetiveria zizanioides (L.) Nash) to a Venezuelan heavy crude oil in soil. Additionally, the plant's potential for stimulating the biodegradation processes of petroleum hydrocarbons was tested under the application of two fertilizer levels. In the presence of contaminants, biomass and plant height were significantly reduced. As for fertilization, the lower fertilizer level led to higher biomass production. The specific root surface area was reduced under the effects of petroleum. However, vetiver was found to tolerate crude-oil contamination in a concentration of 5% (w/w). Concerning total oil and grease content in soil, no significant decrease under the influence of vetiver was detected when compared to the unplanted control. Thus, there was no evidence of vetiver enhancing the biodegradation of crude oil in soil under the conditions of this trial. However, uses of vetiver grass in relation to petroleum-contaminated soils are promising for amelioration of slightly polluted sites, to allow other species to get established and for erosion control.

KEY WORDS:

• phytoremediation
• biodegradation
• petroleum hydrocarbons
• oil contamination
• toxic effect
• fertilizer levels
• vetiver
• root surface area
• tropics

ACKNOWLEDGEMENTS

This work was supported by a grant from the Father-and-Son-Eiselen Foundation (Ulm, Germany).

Notes

¹+ PHC-contaminated soil; − Uncontaminated soil.

²+ 2 vetiver tillers/pot; −No plants.

¹ León et al., 1998.

² Rosales and Villalobos, 2001.