EFFECTS OF NITROGEN AND PHOSPHORUS LEVELS, AND FROND-HARVESTING ON ABSORPTION, TRANSLOCATION AND ACCUMULATION OF ARSENIC BY CHINESE BRAKE FERN (PTERIS VITTATA L.)

Abstract

This hydroponic experiment was conducted to determine the effects of nitrogen (N) and phosphorus (P) levels and frond-harvesting on the effectiveness of arsenic (As)-hyperaccumulator Chinese brake fern (Pteris vittata L.) to remove As from contaminated groundwater collected from south Florida. Three-month old ferns were grown in 38-L plastic tanks (two ferns per tank) containing 30-L of As-contaminated water (130 μg·L⁻¹ As), which was amended with modified 0.25 strength Hoagland's solution #2. Two N (26 or 52 mg·L⁻¹) and two P levels (1.2 and 2.4 mg·L⁻¹) were tested in one experiment, whereas the effect of frond-harvesting was tested in a separate experiment. Initially, N had little effect on plant As removal whereas low P treatment was more effective than high P and As was reduced to <5 μg·L⁻¹ in 28 d compared to 35 d. For well-established ferns, N and P levels had little effect. Reused fern, with or without harvesting the As-rich fronds, took up arsenic more rapidly so the As concentration in the groundwater declined faster (130 to ∼10 μg·L⁻¹ in 8 h). Regardless of the treatments, most As (85–93%) was located in the aboveground tissue (rhizomes and fronds). Frond As concentrations were higher for non-harvested ferns than for ferns where fronds were partially harvested prior to treatment. Conversely, rhizomes accumulated more arsenic in ferns where fronds had been partially harvested. Low-P treatment coupled with reuse of more established ferns with or without harvesting fronds can be used to effectively remove arsenic from contaminated water using P. vittata

KEY WORDS:

• Pteris vittata
• phytofiltration
• arsenic
• nitrogen
• phosphorus
• frond harvesting
• groundwater
• kinetic parameters
• translocation factor
• bioconcentration factor

ACKNOWLEDGMENTS

This research was supported by Florida Power & Light Company and Florida Agricultural Experiment Station. The authors gratefully acknowledge the assistance provided by Diane Rock for fern propagation, Dr. Uttam Saha for GFAAS analysis, and Loretta Satterthwaite for proofreading the manuscript. The valuable comments and suggestions by two anonymous reviewers are also highly appreciated.

Notes

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