Study on the Interaction between the Inclusion Complex of Hematoxylin with β-Cyclodextrin and DNA

Abstract

Ultraviolet-visible (UV-vis) spectra, fluorescence spectra, electrochemistry, and the thermodynamic method were used to discuss the interaction mode between the inclusion complex of hematoxylin with β-cyclodextrin and herring sperm DNA. On the condition of physiological pH, the result showed that hematoxylin and β-cyclodextrin formed an inclusion complex with binding ratio n_hematoxylin:n_{β-cyclodextrin} = 1:1. The interaction mode between β-cyclodextrin-hematoxylin and DNA was a mixed binding, which contained intercalation and electrostatic mode. The binding ratio between β-cyclodextrin-hematoxylin and DNA was n_{β-cyclodextrin -hematoxylin}:n_DNA = 2:1, binding constant was K^⊖ _298.15K = 5.29 × 10⁴ L·mol⁻¹, and entropy worked as driven force in this action.

Keywords:

• Spectrometry
• β-cyclodextrin
• hematoxylin
• inclusion complex
• herring sperm DNA

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30973634) and Postgraduate Innovation Foundation of Southwest University of Science and Technology. The authors are grateful for the apparatus support of the Analytical and Testing Center of Southwest University of Science and Technology.