Concise Synthesis of Triazole-Linked 5′-Peptide-Oligonucleotide Conjugates by Click Chemistry

Abstract

A concise synthesis of oligonucleotide 5′-peptide-conjugates via copper(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition in aqueous solution is described. Synthesis of reagents was accomplished by on-column derivatization of corresponding peptides and oligonucleotides. This method is well suited for the preparation of peptide–oligonucleotide conjugates containing 1,2,3-triazole linkage between the 5′-position of an oligonucleotide and the N-terminus of a peptide.

Keywords:

• Peptides
• oligonucleotides
• conjugates
• click chemistry