Design and Facile Synthesis of New Dinucleotide Cap Analog Containing Both 2′ and 3′-OH Modification on M⁷Guanosine Moiety

Abstract

The first example of the synthesis of new dinucleotide cap analog containing 2^′,3^′-diacetyl group on m⁷guanosine moiety is described. The desired modified cap analog, m^{7,2′,3′–diacetyl}G[5^′]ppp[5^′]G has been obtained by the coupling reaction of triethylamine salt of m^{7,2′,3′–diacetyl}GDP with ImGMP in presence of ZnCl₂ as a catalyst in 62% yield with high purity. The structure of new cap analog has been confirmed by ¹H and ³¹P NMR and mass data.

Keywords:

• Cap analog
• diacetyl cap analog
• 5′-capped mRNA
• dendritic cells