4′-Epi-DNA: A DNA Mimic Containing 4′-hydroxymethyl-α-l-Xylo-Thymidine with Compact Backbone like RNA

ABSTRACT

Synthesis of C4′-epi-DNA containing 3′→ 5″ linkages is reported for the first time. Crystal structure study of the monomer indicated that though the dihedral angle O3′-C3′-C4′-C5″ in this case would be like in RNA, the sugar conformation would remain like that in DNA. The study of the effect of this backbone configuration in DNA with respect to its binding to cDNA and RNA is reported in this note.

GRAPHICAL ABSTRACT

Keywords:

• Antisense Oligonucleotide
• 4′-hydroxymethyl nucleosides C4′-epi-DNA

Acknowledgment

VAK and MF thank CSIR, New Delhi for research funding (BSC0123). SB thanks UGC, New Delhi for research fellowship.