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Nucleosides, Nucleotides & Nucleic Acids Volume 37, 2018 - Issue 12: PROCEEDINGS OF THE 17TH INTERNATIONAL SYMPOSIUM ON PURINE AND PYRIMIDINE METABOLISM IN MAN
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Conference Proceedings

Proteomic analysis of a urinary stone with two layers composed of calcium oxalate monohydrate and uric acid

Kiyoko KanekoLaboratory of Biomedical and Analytical Sciences, Faculty of Pharma-Science, Teikyo University, Tokyo, Japan; Correspondence[email protected]
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,
Mizuho KabeyaLaboratory of Biomedical and Analytical Sciences, Faculty of Pharma-Science, Teikyo University, Tokyo, Japan; View further author information
,
Hirokazu KondoLaboratory of Biomedical and Analytical Sciences, Faculty of Pharma-Science, Teikyo University, Tokyo, Japan; View further author information
,
Tomoko FukuuchiLaboratory of Biomedical and Analytical Sciences, Faculty of Pharma-Science, Teikyo University, Tokyo, Japan; View further author information
,
Noriko YamaokaLaboratory of Biomedical and Analytical Sciences, Faculty of Pharma-Science, Teikyo University, Tokyo, Japan; View further author information
,
Makoto YasudaLaboratory of Analytical Chemistry, Faculty of Pharma-Science, Teikyo University, Tokyo, Japan; View further author information
&
Satoshi YamaguchiDepartment of Urology, Asahikawa Medical College, Asahikawa, JapanView further author information
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Pages 717-723 | Received 15 Jan 2018, Accepted 14 May 2018, Published online: 27 Dec 2018
 
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Abstract

We examined the mechanism of urinary stone formation by analyzing the matrix proteins in a urinary stone with two layers composed of different crystals. Micro-area X-ray spectrometry and infrared spectroscopy revealed calcium oxalate monohydrate in the outside and uric acid in the inside. We also examined the interface. After the outside, inside, and interface parts were separated, proteomic analysis identified 48, 7, and 4 matrix proteins, respectively. Urinary stones with two layers are considered to have grown under different conditions. The matrix proteins in each part differed among the crystal components and may reveal the stone-generating process. The proteins in the interface likely function to enlarge the stone via the addition of different crystals.

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Funding

This work was supported by JSPS KAKENHI [Grant Number 17K11191].

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