In Vitro Incorporation of LNA Nucleotides

Abstract

An LNA modified nucleoside triphosphate 1 was synthesized in order to investigate its potential to act as substrate for DNA strand synthesis by polymerases. Primer extension assays for the incorporation experiments revealed that Phusion High Fidelity DNA polymerase is an efficient enzyme for incorporation of the LNA nucleotide and for extending strand to full length. It was also observed that pfu DNA polymerase could incorporate the LNA nucleotide but it failed to extend the strand to a full length product.

Keywords:

• LNA nucleoside triphosphates
• enzymatic incorporation

Acknowledgments

We greatly appreciate the funding from The Danish National Research Foundation.