Abstract
The synthesis of two pyrene-functionalized 2′-amino-α-L-LNA adenine building blocks is outlined and initial results from thermal denaturation studies are presented.
The Danish National Research Foundation is thanked for financial support.
Notes
a T m values/°C (Δ T m = change in T m value calculated relative to DNA:DNA or DNA:RNA reference duplex) recorded in medium salt buffer ([Na+] = 110 mM, [Cl−] = 100 mM, pH 7.0 (NaH2PO4/Na2HPO4)), using 1.0 μM concentrations of each strand. T m values (°C) of ON1 and ON2 towards DNA strands containing a single mismatch in the central position A/C/G: 18.5/27.0/19.5 and 18.0/27.0/22.0, respectively. T m values (°C) of ON1 and ON2 towards RNA strands containing a single mismatch in the central position A/C/G: 13.5/16.0/14.0 and 16.5/16.0/13.5, respectively.