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Original Articles

Chronic Responses of Daphnia magna Under Dietary Exposure to Leaves of a Transgenic (Event MON810) Bt–Maize Hybrid and its Conventional Near-Isoline

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Pages 993-1007 | Received 18 Mar 2015, Accepted 01 Apr 2015, Published online: 11 Aug 2015

Figures & data

FIGURE 1. Examples of experimental D. magna fed maize leaves. Maize leaf-particles can be seen in the gut of animals. a) D. magna bearing subitaneous eggs. b) D. magna bearing an immature ephippium (protective structure enclosing two dormant eggs). Bars have 2 mm.

FIGURE 1. Examples of experimental D. magna fed maize leaves. Maize leaf-particles can be seen in the gut of animals. a) D. magna bearing subitaneous eggs. b) D. magna bearing an immature ephippium (protective structure enclosing two dormant eggs). Bars have 2 mm.

FIGURE 2. Immuno-detection of MON810’s rCry1Ab. MagicMark™ XP Western Protein Standard (M), positive control (PC - Cry1Ab purified from B. thuringiensis), the three non-transgenic near-isoline plants (NM1, NM2 and NM3) and the three MON810 Bt-maize plants (GM1, GM2 and GM3). Strong immunoreactive bands of ca. 69 kDa and 34 KDa can be distinguished in the GM-maize samples.

FIGURE 2. Immuno-detection of MON810’s rCry1Ab. MagicMark™ XP Western Protein Standard (M), positive control (PC - Cry1Ab purified from B. thuringiensis), the three non-transgenic near-isoline plants (NM1, NM2 and NM3) and the three MON810 Bt-maize plants (GM1, GM2 and GM3). Strong immunoreactive bands of ca. 69 kDa and 34 KDa can be distinguished in the GM-maize samples.

FIGURE 3. Body size of D. magna fed Bt-maize (GM) and near-isogenic maize (NM) leaves. Shaded bands indicate 95% confidence intervals.

FIGURE 3. Body size of D. magna fed Bt-maize (GM) and near-isogenic maize (NM) leaves. Shaded bands indicate 95% confidence intervals.

FIGURE 4. Stage fecundity of D. magna fed Bt-maize (GM) and near-isogenic maize (NM) leaves. Shaded bands indicate 95% confidence intervals.

FIGURE 4. Stage fecundity of D. magna fed Bt-maize (GM) and near-isogenic maize (NM) leaves. Shaded bands indicate 95% confidence intervals.

FIGURE 5. Cumulative fecundity of D. magna fed Bt-maize (GM) and near-isogenic maize (NM) leaves. Shaded bands indicate 95% confidence intervals.

FIGURE 5. Cumulative fecundity of D. magna fed Bt-maize (GM) and near-isogenic maize (NM) leaves. Shaded bands indicate 95% confidence intervals.

FIGURE 6. Ephipum production in D. magna. a) Total ephippia produced in the Daphnia bioassay, for experimental D. magna fed Bt-maize (GM) and near-isogenic maize (NM) leaves. b) Probability of ephippium production among experimental D. magna fed Bt-maize (GM) and near-isogenic maize (NM) leaves. Error bars indicate 95% confidence intervals.

FIGURE 6. Ephipum production in D. magna. a) Total ephippia produced in the Daphnia bioassay, for experimental D. magna fed Bt-maize (GM) and near-isogenic maize (NM) leaves. b) Probability of ephippium production among experimental D. magna fed Bt-maize (GM) and near-isogenic maize (NM) leaves. Error bars indicate 95% confidence intervals.
Supplemental material

ESM3 - Inferential statistics for parameter estimates of fixed effects in best-fit models for all endpoints in the comparison

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ESM2 - Model fit statistics for all endpoints in the comparisons between GM (genetically modified) and NM

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ESM1 - Electronic Supplementary Material and Methods

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