Acute hydrogen peroxide (H₂O₂) exposure does not cause oxidative stress in late-copepodite stage of Calanus finmarchicus

ABSTRACT

Use of hydrogen peroxide (H₂O₂) for removal of salmon lice in the aquaculture industry has created concern that non-target organisms might be affected during treatment scenarios. The aim of the present study was to examine the potential for H₂O₂ to produce oxidative stress and reduce survival in one of the most abundant zooplankton species in Norwegian coastal areas, the copepod Calanus finmarchicus. Copepods were subjected to two 96-hr tests: (1) acute toxicity test where mortality was determined and (2) treated copepods were exposed to concentrations below the No Observed Effect Concentration (0.75 mg/L) H₂O₂ and analyzed for antioxidant enzyme activities, as well as levels of glutathione (GSH) and malondialdehyde (MDA). Compared to available and comparable LC₅₀ values from the literature, our results suggest that C. finmarchicus is highly sensitive to H₂O₂. However, 96-hr exposure of C. finmarchicus to 0.75 mg H₂O₂/L did not significantly affect the antioxidant systems even though the concentration is just below the level where mortality is expected. Data suggest that aqueous H₂O₂ exposure did not cause cellular accumulation with associated oxidative stress, but rather produced acute effects on copepod surface (carapace). Further investigation is required to ensure that aqueous exposure during H₂O₂ treatment in salmon fish farms does not exert adverse effects on local non-target crustacean species and populations. In particular, studies on copepod developmental stages with a more permeable carapace are warranted.

Funding

This work was funded internally by SINTEF, BioTrix, NTNU, Medical University of Gdańsk (ST-40 project), and externally through European Union’s Horizon 2020 GRACE project (grant agreement No 679266).

Additional information

Funding

This work was funded internally by SINTEF, BioTrix, NTNU, Medical University of Gdańsk (ST-40 project), and externally through European Union’s Horizon 2020 GRACE project (grant agreement No 679266).