Oxidative stress-related effects induced by micronized polyethylene terephthalate microparticles in the Manila clam

ABSTRACT

Microplastic (MP) contamination represents a serious threat for marine organisms. Several lab studies demonstrated adverse effects induced by exposure to different MP polymers toward diverse marine species. However, the information regarding toxicity of polyethylene terephthalate (PET) MPs is largely unknown. The present study was aimed at investigating the adverse effects induced by 7-day exposure to two concentrations (0.125 or 12.5 µg/ml) micronized, irregular shaped and variable size PET microparticles (PET-MPs) toward Manila clam (Ruditapes philippinarum). Histological analyses were performed to assess tissue damage on digestive glands, gonads, gut and gills, whereas oxidative stress-related effects, including the concentration of pro-oxidant molecules, activity of antioxidant (superoxide dismutase – SOD, catalase – CAT and glutathione peroxidase – GPx) and detoxifying (glutathione S-transferase – GST) enzymes, as well as levels of lipid peroxidation, were determined in gills and digestive gland. Our results showed that clams ingest and egest micronized PET-MPs, but no marked histological alterations to bivalve tissues occurred. Although PET-MPs did not produce oxidative stress in the digestive gland, these materials significantly altered oxidative status of gills, leading to lipid peroxidation. No apparent clear indication of a weakness of bivalve health status was obtained in this study.

KEYWORDS:

• Manila clam
• microplastics
• oxidative stress
• polyethylene terephthalate (PET)

Author contribution

Marco Parolini: Conceptualization, Writing – Original Draft, Supervision; Beatrice De Felice: Investigation; Stefano Gazzotti, Luisa Annunziata: Investigation; Michela Sugni: Investigation, Renato Bacchetta: Investigation; Marco Aldo Ortenzi: Investigation.