DETECTION OF ANTI-SP100 ANTIBODIES IN PRIMARY BILIARY CIRRHOSIS. COMPARISON OF ELISA AND IMMUNOFLUORESCENCE

Abstract

Primary biliary cirrhosis (PBC) is, which a chronic, autoimmune liver disease. Some patients have antinuclear antibodies anti-Sp100, which are considered to be disease-specific. We compared an enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence (IIF) for detection of anti-Sp100. The sensitivity of anti-Sp100 determined by ELISA and IIF was 44% and 34%, respectively. Specificity was 99% for ELISA and 98% for IIF, respectively. The positive and negative predictive value (PPV, NPV) for anti-Sp100 determined by ELISA were 98%, 60% and 95%, 56% for IIF respectively. IIF required substantial experience in interpreting subjective patterns, whereas ELISA was more sensitive, cheaper, less time consuming, and produced clear-cut quantitative results.

Keywords:

• autoantibody
• ELISA
• immunofluorescence
• primary biliary cirrhosis
• Sp100

ACKNOWLEDGMENTS

The authors thank Professor Barbara Czarnocka, Head of the Department of Biochemistry and Molecular Biology, Medical Centre of Postgraduate Education, for encouraging us to conduct this study, and professor Grażyna Adler for reading the manuscript and for expert advice. This study was supported by grant 502-1-1-25-01/10 from the Medical Center of Postgraduate Education, Warsaw, Poland.

Notes

PBC: primary biliary cirrhosis; PSC: primary sclerosing cholangitis; AIH: autoimmune hepatitis.

PPV: positive predictive value; NPV: negative predictive value.