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Journal of Immunoassay and Immunochemistry Volume 34, 2013 - Issue 4
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Original Articles

PEROXIDASE-LINKED ASSAY FOR DETECTION OF ANTIBODIES AGAINST BOVINE LEUKOSIS VIRUS

Clarissa C. de Castro Laboratory of Virology and Immunology, Universidade Federal de Pelotas, Pelotas, Brazil
,
Cristina F. Nunes Laboratory of Virology and Immunology, Universidade Federal de Pelotas, Pelotas, Brazil
,
Paula F. Finger Laboratory of Virology and Immunology, Universidade Federal de Pelotas, Pelotas, Brazil
,
Bianca S. Siedler Laboratory of Virology and Immunology, Universidade Federal de Pelotas, Pelotas, Brazil
,
Luana Dummer Technology Development Center, Biotechnology Center, Bacteriology Laboratory, Universidade Federal de Pelotas, Pelotas, Brazil
,
Marcelo de Lima Laboratory of Virology and Immunology, Universidade Federal de Pelotas, Pelotas, Brazil
,
Fábio P. L. Leite Technology Development Center, Biotechnology Center, Bacteriology Laboratory, Universidade Federal de Pelotas, Pelotas, Brazil
,
Geferson Fischer Laboratory of Virology and Immunology, Universidade Federal de Pelotas, Pelotas, Brazil
,
Gilberto D'A. Vargas Laboratory of Virology and Immunology, Universidade Federal de Pelotas, Pelotas, Brazil
&
Silvia de O. Hübner Laboratory of Virology and Immunology, Universidade Federal de Pelotas, Pelotas, BrazilCorrespondence[email protected]
 show all
Pages 376-383 | Published online: 16 Jul 2013
 
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Abstract

A peroxidase linked assay (PLA) was designed to screen bovine sera for the presence of specific antibodies against bovine leukosis virus (BLV). Out of 201 samples of bovine sera analyzed, 52.2% were considered positive by PLA, 26.4% by AGID, and 38.9% by ELISA. Western blotting analyses excluded 27 samples found to be positive by PLA. PLA showed 100% of sensitivity when compared with AGID and ELISA. Specificity was 64.8% and 78%, respectively (kappa coefficients were 0.70 and 0.83). These findings indicate that PLA can be used as an alternative method for the diagnosis of BLV infection in cattle.

ACKNOWLEDGMENT

We are grateful to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes) for financial support.

Notes

AGID, agar gel immunodiffusion assay; PLA, peroxidase linked assay; WB, Western blot; ELISA, enzyme-linked immunosorbent assay; +, positive result; −, negative result.

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