Eliciting an antibody response against a recombinant TSH containing fusion protein

ABSTRACT

Designing novel antigens to rise specific antibodies for Thyroid Stimulating Hormone (TSH) detection is of great significance. A novel fusion protein consisting of the C termini sequence of TSH beta subunit and a fusion sequence was designed and produced for rabbit immunization. Thereafter, the produced antibodies were purified and characterized for TSH detection. Our results indicate that the produced antibody is capable of sensitive and specific detection of TSH with low cross reactivity. This study underscores the applicability of designed fusion protein for specific and sensitive polyclonal antibody production and the importance of selecting an amenable region of the TSH for immunization.

KEYWORDS:

• cross reactivity
• detection
• fusion protein
• polyclonal antibody
• TSH

Acknowledgment

The authors wish to thank Tarbiat Modares University for supporting the conduct of this research.