Responses of soil respiration to N fertilization and grazing in a Keerqin sandy grassland in northeast China

Abstract

Nitrogen (N) deposition and grazing (G) are two disturbances of grassland ecosystems and these can greatly affect soil respiration. To date, few studies have investigated this topic in sandy grasslands. Here, we conducted a manipulative experiment in a sandy grassland in Inner Mongolia, China in the year 2013 and 2014, to investigate the effects of N addition and grazing on soil respiration. The results showed that N addition significantly accelerated soil respiration in both 2013 and 2014. The respiration enhancement under N addition varied during the growing season, being larger in early May 2014 (0.40 μmol·m⁻²·s⁻¹) and early July 2014 (1.29 μmol·m⁻²·s⁻¹) than in October 2014 (0.03 μmol·m⁻²·s⁻¹). Grazing plots yielded a lower cumulative soil respiration rate than the control plots (−5,68% in 2013 and −1.07% in 2014), although the difference was not significant. In this study, soil temperature rather than soil moisture was the main factor affecting the temporal variation in soil respiration during the growing season. The inter-plot analysis showed that the soil ammonium nitrogen (NH₄⁺–N) content, C:N ratio, and root biomass were significantly correlated with soil respiration, indicating that soil respiration under N-addition or grazing treatment could be affected by changing soil microhabitat conditions, thus affecting soil respiration.

Keywords:

• Inner Mongolia
• nitrogen application
• CO₂ emission
• C:N ratio
• root biomass

Additional information

Funding

This work was funded by the National Natural Science Foundation of China [31870603, 30800143, 41401262] and the Natural Science Foundation of Liaoning Province [No. 2019-MS-341].