Hepatotoxicity and nephrotoxicity in rats induced by carbon tetrachloride and the protective effects of Teucrium polium and vitamin C

Abstract

This work investigated the protective effects of Teucrium polium (T. polium) and vitamin C (Vit C) against carbon tetrachloride (CCl₄) induced hepatotoxicity and nephrotoxicity in rats. T. polium reduced the Fer reduced antioxidant power (FRAP) (IC₅₀ = 0.89 mg/ml) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) (IC₅₀ = 0.049 µg/ml) than Vit C, FRAP (IC₅₀ = 0.71 mg/ml) and DPPH (IC₅₀ = 0.029 µg/ml). Male albino Wistar rats were divided into six groups: Group I was used as controls, Group II received CCl₄ in olive oil (0.5 ml/kg) by gavage, Group III received CCl₄ in olive oil (0.5 ml/kg) by gavage after 3 d of receiving T. polium (5 g/l), orally, Group IV received T. polium (5 g/l) alone, by gavage, for 7 d, Group V received CCl₄ in olive oil (0.5 ml/kg) by gavage after 3 d of receiving Vit C (250 mg/kg) by gavage and Group VI received Vit C (250 mg/kg) alone by gavage. CCl₄ showed an increase of serum hepatic and renal markers aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, and creatinine. Moreover, we noted an increase of lipid peroxidations and a decrease in antioxidants enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) of CCl₄ rats compared to controls. The pretreatment with (200 mg/kg) of T. polium and with Vit C (250 mg/kg) by gavage, for 7 d, displayed their ability to protect against oxidative damage and biochemical changes induced by CCl₄. Our results were in accordance with histopathological observations.

Graphical Abstract

Keywords:

• Carbon tetrachloride
• oxidative stress
• vitamin C
• Teucrium polium

Acknowledgment

The authors would like to thank Mr. Wassim Hariz, English professor at the Faculty of Science of Sfax, for having proofread the manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the research unit of the Laboratory Animal Ecophysiology, University of Sfax, and the Laboratory of Histology-Embryology, Faculty of Medicine, Sfax.