Figures & data
Figure 1. (A) Flow cytometric analysis of IK-CD133+ cells labeled with FITC. (B) Flow cytometric analysis of IK-CD133+ cells in negative control. (C) Flow cytometric analysis of IK-CD133+ cells after cell purification by MACSR Technology.
Figure 2. (A) Representative cell sphere of IK-CD133+ cells. (B) The proliferation ability of IK-CD133+ cells and IK-CD133- cells. (C) The ability of IK-CD133+ cells and IK-CD133- cells to form colonies. (D) The apoptosis rate of IK-CD133+ cells and IK-CD133- cells. * P < 0.05.
Figure 3. (A) The location and relative expression of Notch1 in IK-CD133+ cells and IK-CD133- cells by immunofluorescence. (B) The expression of Notch1 in IK-CD133+ cells and IK-CD133- cells. IDV is the abbreviation for “integrated density values.” * P < 0.05.
Figure 4. The proliferation ability inhibition in IK-CD133+ cells with different concentrations of AG1478 (A) and DAPT (B). * P < 0.05, ** P < 0.01.
Figure 5. The apoptotic rate (A), the proportion of G1 stage cells (B), the numbers of trans-membrane cells (C) and the expression of HES-1, NICD, Notch1, pMAPK and pAKT protein (D) in IK-CD133+ cells and HEC-1A cells treated with 10 μmol/L AG1478, 10 μmol/L DAPT and 10μmol/L AG1478+ 10 μmol/L DAPT. * P < 0.05, ** P < 0.01.
Figure 6. (A) The nude mice transplanted tumor established from IK-CD133+ cells and IK-CD133- cells in vivo. Left tumor is derived from IK-CD133+ cells, right tumor IK-CD133- cells. (B) Tumor growth curves were measured after the injection of IK-CD133+ cells and IK-CD133- cells. Tumor volume was calculated every 5 d. **P < 0.01 vs. IK-CD133- cells (n = 8).
