Figures & data
Figure 1. Negative Correlation between ANRIL and miR-125a in NPC tumor tissues. (A and B) qRT-PCR analysis of ANRIL and miR-125a expression in NPC patient tumor tissue samples (n = 35) and normal nasopharyngeal tissue samples (n = 35). (C) The ANRIL level and miR-125a expression had a negative correlation in NPC tumor tissues. *P < 0.05 vs. NC.
Figure 2. ANRIL expression is upregulated and miR-125a expression is downregulated in NPC cell lines. (A) ANRIL expression was examined by qRT-PCR analysis in 5–8F, CNE1, CNE2 and HONE1 cells or HNEpC. (B) qRT-PCR analysis was conducted to determine the expression of miR-125a in 5–8F, CNE1, CNE2 and HONE1 cells or HNEpC. *P < 0.05 vs. HNEpC.
Figure 3. ANRIL knockdown inhibits proliferation of NPC cell lines CNE2 and HONE1. CNE2 and HONE1 cells were transfected with si-control or si-ANRIL. (A and B) The level of ANRIL was detected by western blot in CNE2 and HONE1 cells. (C and D) MTT assay was performed to detect the cell viability at 24, 48 and 72 h after transfection in CNE2 and HONE1 cells. *P < 0.05 vs. si-control.
Figure 4. ANRIL downregulation induces apoptosis, and enhances radiosensitivity in NPC cell lines CNE2 and HONE1. CNE2 and HONE1 cells were transfected with si-control or si-ANRIL. (A and B) Cell apoptosis was determined by flow cytometry analysis at 48 h after transfection in CNE2 and HONE1 cells. (C) The colony survival was determined by clonogenic assay in CNE2 and HONE1 cells treated with IR (0, 2, 4, 6 and 8 Gy). *P < 0.05 vs. si-control.
Figure 5. ANRIL represses the miR-125a expression in CNE2 and HONE1 cells. (A) Putative miR-125a binding sequence of ANRIL was shown. (B) The relative luciferase activity was detected in CNE2 and HONE1 cells co-transfected with miR-125a mimic or miR-control and wt or mut ANRIL. (C and D) qRT-PCR analysis of miR-125a expression in si-ANRIL or pcDNA-ANRIL transfecting CNE2 and HONE1 cells. *P < 0.05 vs. controls.
Figure 6. Knockdown of ANRIL inhibits proliferation, induces apoptosis, and enhances radiosensitivity in NPC cells through negatively regulating miR-125a expression. CNE2 and HONE1 cells were were transfected with miR-125a or co-transfected with pcDNA-ANRIL and miR-125a. (A) MTT assay was performed to determine the cell viability of CNE2 and HONE1 cells. (B) Flow cytometry analysis was performed to measure the cell apoptosis of CNE2 and HONE1 cells at 48 h after transfection. (C) Clonogenic assay was conducted to determine the colony survival in CNE2 and HONE1 cells treated with IR (0, 2, 4, 6 and 8 Gy). *P < 0.05 vs. controls.
