https://orcid.org/0000-0002-6368-1937
Figures & data
Figure 1. Expression of miR-219a-1 in patients with colon cancer and cell lines. The level of miR-219a-1 was detected by qRT-PCR in patients with colon cancer and healthy individuals (a), and different cell lines (b), including normal colon cell line FHC, colon cancer cell line SW480 and SW620. P = .0423 versus healthy control, t-test; * P < .05 versus FHC, one-way ANOVA followed by SNK test
Figure 2. miR-219a-1 inhibits SW480 and SW620 cell proliferation, invasion and migration. (a) miR-219a-1 mimics inhibited SW480 and SW620 cell proliferation. (b) miR-219a-1 inhibitor induced SW480 and SW620 cell proliferation. (c) miR-219a-1 mimics or inhibitor was transfected into SW480 or SW620 cells, and then transwell assay was performed. Representative images of SW480 and SW620 cell invasive ability after cells transfection. (d) Cell invasiveness was quantified by counting cells that passed through the Matrigel membrane, using a light microscope (×200). (e) Wound-healing assays were performed after SW480 and SW620 cells transfected with miR-219a-1 mimics or inhibitor. * or # P < .05, ** or ## P < .01, *** or ### P < .001
Figure 3. MEMO1 is a potential target of miR-219a-1 (a) Human MEMO1 3ʹ-UTR fragment containing wide-type (WT) or mutated (MUT, indicated in red) miR-219a-1-binding sequence. (b) Luciferase reporter assays in SW480 cells, with cotransfection of pMEMO1-WT or pMEMO1-MUT and miR-219a-1 mimics or miR-NC as indicated. Luciferase activities were measured 48 h post-transfection. * P < .05, ** P < .01, *** P < .001
Figure 4. miR-219a-1 inhibits MEMO1 expression in SW480 and SW620 cells. (a) MEMO1 mRNA level in SW480 and SW620 cells transfected with miR-219a-1 mimics or inhibitor was analyzed by qRT-PCR. (b) MEMO1 protein level in SW480 and SW620 cells transfected with miR-219a-1 mimics or inhibitor was analyzed by Western-blot. * P < .05, ** P < .01, *** P < .001
Figure 5. MEMO1 expression was inversely correlated with miR-219a-1 in patients with colon cancer. (a) MEMO1 mRNA expression in patients with colon cancer (n = 17) and healthy individuals (n = 20) was detected by qRT-PCR. (b) A negative correlation was found between mRNA expression of MEMO1 and miR-219a-1 in patients with colon cancer (r = −0.8575; P < .0001, Pearson’s correlation)
