Figures & data
Figure 1. Effect of thapsigargin on the expression of LIP and T-Ag. BsB8 cells were treated with thapsigargin (TG) and then analyzed by Western blot for expression of T-Ag (A) and LIP (B) as described in Materials and Methods. The loading control was α-tubulin. The left-hand lane contains the molecular weight markers (MW). The effect of TG on BsB8 cell viability was measured by MTT assay (C).
Figure 2. Effect of overexpression of LIP and LIP siRNA on T-Ag expression. (A) BsB8 cells were transfected with different amounts of expression plasmid for LIP as indicated and T-Ag expression analyzed by Western blot. The loading control was α-tubulin. The left-hand lane contains the molecular weight markers (MW). (B) BsB8 cells were transfected with expression plasmid for LIP and transduced with adenovirus vectors as indicated and T-Ag expression analyzed by Western blot. The loading control was α-tubulin. The left-hand lane contains the molecular weight markers (MW).
Figure 3. Effect of MG118 on LIP downregulation of T-Ag expression. BsB8 cells were transfected with different amounts of expression plasmid for LIP in the absence and presence of MG115 as indicated and T-Ag expression analyzed by Western blot. The loading control was α-tubulin.
Figure 4. Immunoprecipitation(IP)/Western blot of LIP and T-Ag. BsB8 cells were transfected with expression plasmid for LIP, whole cell extract (WCE) prepared and: (A) IP performed with antibody to T-Ag or nonimmune mouse serum (NMS) followed by Western blot for LIP and T-Ag. The left-hand lane contains the molecular weight markers (MW). (B) IP performed with antibody to LIP or nonimmune mouse serum (NMS) followed by Western blot for LIP and T-Ag. The left-hand lane contains the molecular weight markers (MW).
