Differential perturbation of the interstitial cystitis-associated genes of bladder and urethra in rat model

Figures & data

Figure 1. A workflow of this study.

Table 1. Cystometric parameters in conscious, unrestrained Sprague-Dawley rats in the sham and IC groups. Table 1. Cystometric parameters (including pressure and volume parameters) in awake rats subjected to sham-operation or intravesical PS/LPS-treated rats (IC rats).

	BP (cmH2O)	TP (cmH2O)	MP (cmH2O)	BC (mL)	MV (mL)	RV (mL)	MI (min)
Sham	16.14 ± 1.16	32.16 ± 2.04	74.49 ± 5.71	1.86 ± 0.18	1.86 ± 0.18	0	9.99 ± 0.91
IC	18.87 ± 2.89	38.10 ± 3.76	60.90 ± 4.68	1.31 ± 0.14#	1.17 ± 0.10#	0.15 ± 0.09	6.22 ± 0.83#

BP, basal pressure; TP, threshold pressure; MP, micturition pressure; BC, bladder capacity; MV, micturition volume; RV, residual volume; MI, micturition interval; IC, interstitial cystitis; Results are expressed as the mean ± standard error. Pressure parameters were expressed by intravesical pressure.

# p < 0.05,

Figure 2. Identification of differentially expressed genes (DEGs) in bladder or urethra obtained from IC rats compared with sham controls. (A) A heatmap showing DEGs (B) Diagrams indicating IC-specific DEGs in bladder or urethra (upper), and bladder- or urethra-specific DEGs associated with IC (bottom).

Table 2. (A) List of 5 common genes in the comparison of IC vs. Sham controls, (B) List of 4 common genes in the comparison of bladder vs. urethra.

A
Symbol	Description	IC/Sham (Bladder)	IC/Sham (Urethra)
Col7a1	collagen, type VII, α 1	−0.65	−0.97
Itga7	integrin, α 7	0.81	−0.6
Serpina3n	serine (or cysteine) peptidase inhibitor, clade A, member 3N	−0.88	−0.74
Slc25a24	solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 24	0.7	1.14
Slit3	slit homolog 3 (Drosophila)	−0.86	0.99
B
Symbol	Description	Bladder/Urethra (Sham)	Bladder/Urethra (IC)
Fmo5	flavin containing monooxygenase 5	0.77	0.99
Itga7	integrin, α 7	−0.75	0.67
Lcp1	lymphocyte cytosolic protein 1	0.73	0.84
Mettl7b	methyltransferase like 7B	−0.84	0.76

Figure 3. (A) Enriched cellular processes perturbed in IC rats by PS and LPS treatment. Representative DEGs of “response to abiotic stimulus” and “epithelial cell differentiation” were indicated in orange boxes. Blue, bladder specific; Orange, urethra-specific DEGs. (B) Differentially enriched cellular processes changed sham or IC specifically. Gray, sham control specific; Green, IC specific DEGs.

Figure 4. (A) Bladder specific DEGs, (B) Urethra specific DEGs, (C) Expression patterns obtained from RNA-Sequencing data of 10 genes.

Figure 5. Further validation using qRT-PCR analysis.