Figures & data
Figure 1. DKK-1 expression in PDAC tissue at mRNA level. (A-C). Expression of DKK-1 was significantly increased in PDAC tumor tissues compared with the adjacent non-tumor tissues (ANT) in GSE 15471, GSE 28735 and GSE 71729. (D) The result of qRT-PCR in Ren Ji cohort 1 showed that the expression of DKK-1 was elevated in PDAC tissue compared with normal pancreas (NP).
Figure 2. DKK-1 expression in PDAC tissue at protein level. (A) Western blot of 8 PDAC cell lines and 1 nonmalignant HPDE6-C7 cells and the quantification of western blot demonstrated that expression of DKK-1 was increased in PDAC cell lines. (B) Typical imagine of DKK-1 expression in PDAC tissue microarray by IHC. (C) In Ren Ji cohort 2, the rate of high DKK-1 expression was significantly higher in PDAC group compared with NP group.
Table 1. Correlations between DKK1 expression and clinicopathologic features in Ren Ji cohort 2 patients with pancreatic ductal adenocarcinoma (PDAC).
Figure 3. DKK-1 expression is associated with survival in PDAC patients. A. Kaplan-Meier survival curves demonstrated that patients with high expression of DKK-1 lived significantly shorter than those with low expression of DKK-1 in GSE28735. B. Data from TCGA-PAAD database showed that patients with high expression of DKK-1 had shorter OS and RFS than the patients in low DKK-1 group. C-D. In Ren Ji cohort 1 and 2, we defined the DKK-1 expression based on the results of qRT-PCR and IHC analysis, the Kaplan-Meier survival analysis suggested that high expression of DKK-1 is positive correlated with shorter OS in patients with PDAC. E. In Ren Ji cohort 2, we found that patients with high expression of DKK-1 lived shorter independent of TNM stage.
Table 2. Univariable and multivariable analyses of prognostic parameters for survival in Ren Ji cohort 2 patients with pancreatic ductal adenocarcinoma (PDAC).