Figures & data
Figure 1. Knockdown of NEDD4 in liver cancer cells. A. mRNA level of NEDD4 was detected by q-PCR in liver cancer cell lines after transfected with NEDD4 siRNAs. B. Protein level of NEDD4 was detected by Western blotting analysis in liver cancer cells after transfected with NEDD4 siRNAs. * P<0.05 vs control.
Figure 2. Knockdown of NEDD4 inhibited liver cancer cells growth and promoted apoptosis. A. MTT assay was performed in liver cancer cells after 48 h and 72 h of NEDD4 depletion. B. Cell apoptosis in NEDD4 siRNA transfected liver cancer cells was accessed by Flow cytometry. Lower penal: Quantitative results are illustrated. * P<0.05 vs control.
Figure 3. Knockdown of NEDD4 inhibited cell migration and invasion in liver cancer cells. A. Left panel: Invasion assay was conducted to measure the invasive capacity of liver cancer cells after NEDD4 depletion. Right panel: Quantitative results are illustrated for panel A. B. Left panel: Wound healing assays were used to detect the motility in liver cancer cells after NEDD4 depletion. Right panel: Quantitative results are illustrated for panel B. * P<0.05 vs control.
Figure 4. Knockdown of NEDD4 inhibited LATS1 expression. A. Upper panel: Western blotting analysis was used to detect the expression of LATS1 and its target pAkt in QGY7703 liver cancer cell line after NEDD4 depletion. Lower panel: Quantitative results are illustrated for panel A. B. Upper panel: Western blotting analysis was performed to measure the expression of LATS1 and pAkt in SMMC7721 cells after NEDD4 depletion. Lower Panel: Quantitative results are illustrated for panel B. * P<0.05 vs control.
Figure 5. Overexpression of NEDD4 triggered cell proliferation and abrogated cell apoptosis. A. Cell proliferation was detected by MTT assay in both liver cancer cells after 48 h and 72 h of NEDD4 siRNA transfection. * P<0.05 vs control. B. Cell apoptosis was measured by Flow cytometry.
Figure 6. Overexpression of NEDD4 enhanced cell invasion and migration in liver cancer cells. A. Left panel: Invasion assay was conducted by Transwell chambers assay to measure the invasive capacity of liver cancer cells after NEDD4 overexpression. Right panel: Quantitative results are illustrated for panel A. B. Left panel: Wound healing assays were used to detect the motility. Right panel: Quantitative results are illustrated for panel B. * P<0.05 vs control.
Figure 7. Overexpression of NEDD4 increased LATS1 expression. A. Upper panel: Western blotting analysis was used to detect the expression of LATS1 and pAkt in QGY7703 liver cancer cell line after NEDD4 cDNA transfection. Lower panel: Quantitative results are illustrated for panel A. B. Upper panel: The expression of LATS1 and pAkt in SMMC7721 cells were detected by Western blotting after NEDD4 overexpression. Lower Panel: Quantitative results are illustrated for panel B. * P<0.05 vs control.
