Figures & data
(a) A schematic diagram of the purification protocol. (b) Proteins associated with Ntr1-TAP, Ntr2-TAP, Brr2-TAP and Gpl1-TAP were isolated from cycling S. pombe by tandem affinity purification, separated by SDS-PAGE and visualized by silver staining. Molecular weight marker (M) is indicated on the left. (c) List of selected proteins co-purifying with S. pombe Ntr1-TAP, Ntr2-TAP, Brr2-TAP and Gpl1-TAP identified by mass spectrometry. For a full list of identified proteins, see Table S1.
Supplemental material