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Identification of proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1 in the fission yeast Schizosaccharomyces pombe

, , , , , , , ORCID Icon, ORCID Icon & ORCID Icon show all
Pages 1532-1536 | Received 13 Apr 2019, Accepted 30 May 2019, Published online: 20 Jun 2019

Figures & data

Figure 1. Identification of proteins co-purifying with S. pombe Ntr1-TAP, Ntr2-TAP, Brr2-TAP and Gpl1-TAP.

(a) A schematic diagram of the purification protocol. (b) Proteins associated with Ntr1-TAP, Ntr2-TAP, Brr2-TAP and Gpl1-TAP were isolated from cycling S. pombe by tandem affinity purification, separated by SDS-PAGE and visualized by silver staining. Molecular weight marker (M) is indicated on the left. (c) List of selected proteins co-purifying with S. pombe Ntr1-TAP, Ntr2-TAP, Brr2-TAP and Gpl1-TAP identified by mass spectrometry. For a full list of identified proteins, see Table S1.
Figure 1. Identification of proteins co-purifying with S. pombe Ntr1-TAP, Ntr2-TAP, Brr2-TAP and Gpl1-TAP.
Supplemental material

Supplemental Material

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