Circ_0000003 promotes the proliferation and metastasis of non-small cell lung cancer cells via miR-338-3p/insulin receptor substrate 2

Figures & data

Table 1. Sequences used for qRT-PCR.

circ_0000003	F: CACAAGCTCTTTGACTGGGC
	R: GTGTGGCTCTGAGGTCCTTG
miR-338-3p	F: ATCCGTGAAGTTGTTCGTGG
	R: TATGGTTGTAGAGGACTCCTTGAC
U6	F: CTCGCTTCGCRCAGCACA
	R: AACGCTTCACGAATTTGCGT
IRS2	F: GGGGACAAGAAGGTCATCGC
	R: CGAAGGTACTTCCTGGGGTTA
β-actin	F: CCTGTACGCCAACACAGTGC
	R: ATACTCCTGCTTGCTGATCC

Note: F stands for forward; R stands for reverse; RT stands for reverse transcription.

Figure 1. The expressions of circ_0000003, miR-338-3p and IRS2 mRNA in NSCLC.

Table 2. Correlations between circ_0000003 expression and clinical characteristics in NSCLC patients.

Pathological indicators	Number of patients	Relative expression of hsa-circ-0000003		Chi-square value	P value
		High expression	Low expression
All cases	43	24	19
Age (years)
≤60	21	10	11	1.1177	0.2904
>60	22	14	8
Gender
Male	25	12	13	1.4786	0.2239
Female	18	12	6
Smoking
Smokers	24	15	9	0.9845	0.3210
Never smokers	19	9	10
Histology
Squamous	16	10	6	0.4619	0.4967
Adenocarcinoma	27	14	13
Tumor size(d/cm)
<3	23	11	12	1.2794	0.2580
≥3	20	13	7
Lymph node metastasis
No	14	10	14	4.4080	0.0358
Yes	19	14	5
TNM stage
I–II	19	7	12	4.9682	0.0258
III–IV	24	17	7

Figure 2. Circ_0000003 can promote the proliferation, migration and invasion of NSCLC cells and inhibit apoptosis.

Figure 3. Circ_0000003 sponged miR-338-3p in NSCLC and negatively regulated its expression level.

Figure 4. Circ_0000003 can regulate the proliferation and apoptosis of NSCLC cells by sponging miR-338-3p.

Figure 5. Circ_0000003/miR-338-3p axis modulates the invasion and invasion of NSCLC cells.

Figure 6. The knockdown of circ_0000003 can impede the tumor growth in vivo.

Data Availability Statement

The data used to support the findings of this study are available from the corresponding author upon request.