https://orcid.org/0000-0002-0856-1334
Figures & data
Figure 1. mRNA expression of miR-203 and target gene DDOST were detected in telogen and anagen skin tissue of Cashmere goats. In this figure, detection of miR-203 in telogen and anagen(Figure 1a);detection of DDOST and NAE1 mRNA in telogen and anagen(Figure 1b). The results showed that the expression level of miR-203 was reversed to the expression level of DDOST and NAE1. All data in this figure represent the mean ±SEM of three independent experiments, **P < 0.01
Figure 2. Protein expression of DDOST and NAE1 was detected in telogen and anagen skin tissue of Cashmere goats. In this figure, detection of DDOST and NAE1 by Western blot in telogen and anagen. All data in this figure represent the mean ±SEM of three independent experiments, *p < 0.05
Figure 3. Luciferase reporter assay. In this figure, luciferase reporter vectors of DDOST were constructed, and it has been assayed by sequencing. The sequencing results confirmed that luciferase reporter vectors were constructed successfully. mut, mutant; WT, wild type
Figure 4. The targets of miR-203. MiR-203 binding site on the target genes 3ʹ UTR is shown, together with its homology across species. Target points were detected by Dual-Luciferase Reporter gene assay. In the experiment, transfection was divided into three groups, including WT group, MT group, and psicheck-2 group (Psi). Mimics (mi) and NC were, respectively, transfected with WT and mut. Detection of expression of DDOST and NAE1 in three groups by Dual-Luciferase Reporter gene detection system. NC, negative control.(Figure 4a-b) All data in this figure represent the mean ±SEM of three independent experiments, *p < 0.05, **P < 0.01
Figure 5. Over-expression of miR-203 in HEK-293 T cells. The relative expressions of miR-203 in miR-203 over-expression and negative control were detected by qPCR(Fig5a);the relative expressions of DDOST in miR-203 over-expression and negative control were detected by qPCR(Fig5b-c). The protein expressions of DDOST and NAE1 in miR-203 over-expression and negative control were detected by western blot. mi, NC, and ZC, respectively, represent the cells transfected with miR-203, miR-203 negative control and the blank group(Fig5d-e). All data in this figure represent the mean ±SEM of three independent experiments, **P < 0.01
