Figures & data
Figure 1. Level of circSMARCA5 in colon cancer tissues. (a) Expression levels of circSMARCA5 in colon cancer clinical specimens (n = 35) and the corresponding non-tumor tissues (n = 35) were measured by RT-qPCR. (b) RT-qPCR analysis of circSMARCA5 expression in SW480 and SW620 cells transfected with circSMARCA5 overexpressing plasmid or vector. ** p < 0.01
Figure 2. Impacts of circSMARCA5 on colon cancer growth. SW480 and SW620 cells were transfected with circSMARCA5 overexpressing plasmid/Vector. (a) Viability assessed by MTT assay 48 h after transfection. (b) The growth curves evaluated by using MTT assay. (c) Colony formation assessed by crystal violet. (d) Apoptosis assessed by flow cytometry. Western blot of total protein extracted from transfected cells were probed for (e) p53, p21, CyclinD1, (f) Bax and cleaved-caspase-3. ** p < 0.01, *** p < 0.001
Figure 3. Impacts of circSMARCA5 on colon cancer growth in vivo. BALB/c nude mice were subjected to subcutaneous injection of SW480 cells that were transfected with circSMARCA5 overexpressing plasmid/vector. (a) Tumor volume and (b) weight was measured. * p < 0.05,** p < 0.01, *** p < 0.001
Figure 4. Impacts of circSMARCA5 on colon cancer migration and invasion. SW480 and SW620 cells were transfected with circSMARCA5 overexpressing plasmid. (a) Migration capacity assessed by Transwell assay. (b) Invasion capacity assessed by Matrigel Transwell assay. ** p < 0.01
Figure 5. Direct binding between circSMARCA5 and miR-552. (a) RT-qPCR analysis of miR-552 expression in SW480 and SW620 cells transfected with circSMARCA5 overexpressing plasmid or vector. (b) Binding sites between circSMARCA5 and miR-552. (c) Luciferase reporter plasmids carrying the wild-type (WT) or mutant (MUT) sequence of circSMARCA5 (circSMARCA5WT or circSMARCA5MUT) were transfected into 293 cells with miR-552 mimic (or mimic NC). The 293 cells were harvested 24 h later for luciferase reporter assay. ** p < 0.01
Figure 6. Participation of miR-552 in circSMARCA5-regulated colon cancer cell growth. (a) RT-qPCR analysis of miR-552 expression in SW480 and SW620 cells transfected with miR-552 mimic (or mimic NC). SW480 and SW620 cells were transfected with circSMARCA5 overexpressing plasmid (or vector) and miR-552 mimic (or mimic NC). (b) Viability assessed by MTT assay 48 h after transfection. (c) The growth curves evaluated by using MTT assay. (d) Colony formation assessed by crystal violet. (e) Apoptosis assessed by flow cytometry. Western blot of total protein extracted from transfected cells were probed for (f) p53, p21, CyclinD1 (g) Bax and cleaved-caspase-3. * p < 0.05, ** p < 0.01, *** p < 0.001
Figure 7. Participation of miR-552 in circSMARCA5-regulated colon cancer cell migration and invasion. SW480 and SW620 cells were transfected with circSMARCA5 overexpressing plasmid (or vector) and miR-552 mimic (or mimic NC). (a) Migration capacity assessed by Transwell assay. (b) Invasion capacity assessed by Matrigel Transwell assay. * p < 0.05, ** p < 0.01
Figure 8. Impacts of circSMARCA5 on Wnt and YAP1 pathways. SW480 and SW620 cells were transfected with circSMARCA5 overexpressing plasmid (or vector) and miR-552 mimic (or mimic NC). (a-b) Western blot of total protein extracted from transfected cells were probed for wnt3a, β-catenin and phosphorylated YAP1 (p-YAP1) and total YAP1 (t-YAP1). *** p < 0.001
Data availability
The dataset(s) supporting the conclusions of this article is(are) included within the article.