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Review

A Survey and Critical Evaluation of Isolation, Culture, and Cryopreservation Methods of Human Amniotic Epithelial Cells

, , , , , , & show all
Pages 655-673 | Received 21 Oct 2021, Accepted 10 Dec 2021, Published online: 15 Mar 2022

Figures & data

Figure 1. A comparison of hAECs isolation protocols. AM, amniotic membrane; Cells (m)/g, cells (in million) per gram of tissue; cells (M)/amnion, cells (in million) per amnion; Cg, collagenase; CF, calcium-free; CHM, cell heterogeneity markers; CMF, calcium magnesium-free; CM, clinical method; ESCM, epithelial cell surface markers; GS, gentle shaking; IM, immunogenicity markers; NA, not available; P/S, penicillin/ streptomycin; PM, pluripotency markers; SCM, stem cell markers for non-differentiated cells; SW, shaking water bath; TM, traditional method; VS, vigorous shaking. *Original protocols.

Figure 1. A comparison of hAECs isolation protocols. AM, amniotic membrane; Cells (m)/g, cells (in million) per gram of tissue; cells (M)/amnion, cells (in million) per amnion; Cg, collagenase; CF, calcium-free; CHM, cell heterogeneity markers; CMF, calcium magnesium-free; CM, clinical method; ESCM, epithelial cell surface markers; GS, gentle shaking; IM, immunogenicity markers; NA, not available; P/S, penicillin/ streptomycin; PM, pluripotency markers; SCM, stem cell markers for non-differentiated cells; SW, shaking water bath; TM, traditional method; VS, vigorous shaking. *Original protocols.

Table 1. A comparison of hAECs culture protocols.

Table 2. Commercially available human amniotic epithelial cell lines.

Table 3. Comparison of cryopreservation media.

Table 4. Clinical trials using hAECs as a method of intervention.