MiR-17- 5p/RRM2 regulated gemcitabine resistance in lung cancer A549 cells

Figures & data

Table 1. The sequences of primers of genes.

Gene’s Name	Primer	Sequence
U6	forward primer	5”-agagaagattagcatggcccctg-3”
	reverse primer	5”-agtgcagggtccgaggtatt-3”
	RT primer	5”-cgtatccagtgcagggtccgaggtattcgcactggatacgacaaaagc-3”
miR-17-5p	forward primer	5”-gcgcaaagtgcttacagtgc-3”
	reverse primer	5”-agtgcagggtccgaggtatt-3”
	RT primer	5”-gtcgtatccagtgcagggtccgaggtattcgcactggatacgac-3”
GAPDH	forward primer	5”-caggaggcattgctgatgat-3”
	reverse primer	5”-gaaggctggggctcattt-3”
RRM2	forward primer	5”-agtggaaggcattttcttttcc-3”
	reverse primer	5”-gcaaaatcacagtgtaaaccct-3”

Figure 1. pmirGLO vector structure map and the expression of miR-17-5p affects the effects of gemcitabine on the growth and proliferation of A549/G+ and A549/G- cells.

Table 2. The sequence of RRM2 gene and RRM2 mutation gene.

Gene Name	Sequence
RRM2 WT	gaagtgttacgagctccaccatgaattgtccgtaatgttcattaacagcatctttaaaactgtgtagctacctcacaaccgtgtaagctgggttttatattacatggcatttcctgttttgtaacactttgcagttctttcttatggtattttcccgactttaatgtttacttaaatataaacctggcactttacaaacaaataaacattgtttgtactcatagtagagccaggagcggtggctcatgcctgtaatcccagcactttggtaggccgaggcgggtggatcactgcaggtatgtgctaccacacctggctaatttttgtatttttagtagagatggagtttca cctgcaggtcaggctgg
RRM2 MUT non-seed	gaagtgttacgagctccaccatgaattgtccgtaatgttcattaacagcatctttaaaactgtgtagctacctcacaaccgtgtaagctgggttttatattacatggcatttcctgttttgtaatctggggcagttctttcttatggtattttcccgactttaatgtttacttaaatataaacctgatctgggccaaacaaataaacattgtttgtactcatagtagagccaggagcggtggctcatgcctgtaatcccaatctgggggtaggccgaggcgggtggatcactgcaggtatgtgctaccacacctggctaatttttgtatttttagtagagatggagtttcacctgcaggtcaggctgg
RRM2 MUT2	gaagtgttacgagctccaccatgaattgtccgtaatgttcattaacagcatctttaaaactgtgtagctacctcacaaccgtgtaagctgggttttatattacatggcagggttgaggggagcctctgggacagttctttcttatggtattttcccgactttaatgtttacttaaatataaacctgatctgggccaaacaaataaacattgtttgtactcatagtagagccaggagcggtggctccgattgagccgtttcatctgggagtaggccgaggcgggtggatcactgcaggtatgtgctaccacacctggctaatttttgtatttttagtagagatggagtttcacctgcaggtcaggctgg
RRM2 MUT4	gaagtgttacgagctccaccatgaattgtccggccgaggtcggcctcatcgtgggaaaactgtgtagctacctcacaaccgtgtaagctgggttttatattacatggcagggttgaggggagcctctgggacagttctttcttatggtattttcccgactttaatgtttacttaaatataaacctgatctgggccaaacaaataaacattgtttgtactcatagtagagccaggagcggtggctccgattgagccgtttcatctgggagtaggccgaggcgggtggatcactgcaggtatgtgctaccctcttgaatgccgggggagcgggggagtagagatggagtttcacctgcaggtcaggctgg

Figure 2. The results of cell cycle and its relative proteins expression.

Figure 3. The results of dual-luciferase reporter gene experiment, the expression of RRM2 after transfection of miR-17-5p mimics and inhibitor and growth & clone formation of treated by siRRM2.

Figure 4. The cell cycle changes, the protein changes of RRM2, and cell cycle relative protein.

Figure 5. The protein expression difference PTEN, p-PTEN, and PI3K under different conditions.

Data availability statement

We confirm that the data supporting the findings of this study are available within the supplementary materials.