Figures & data
Figure 1. Pig kidneys pre- and post-decellularization and collagen-IV staining of glomerular structures. Gross image of pig kidney pre- (A) and post-decellularization (B), scale bar 1 cm. Collagen-IV immunohistochemical staining of basement membranes (C–H). Pig kidney histology before decellularization (C–E) and after (F–H); arrow heads point to acellular glomerular vascular tufts (G,H). Scale bar (C–H) 100 μm.
Figure 2. Fluorescent 10 μm polystyrene microspheres (FluoSpheres®) with antegrade perfusion in renal artery (red), (A) visualizing afferent arteriole and glomerulus and retrograde perfusion in ureter (green), (B). Scale bar (A, B) 1 cm. Ten μm polystyrene FluoSpheres® (arrowhead) injected retrograde in the ureter and reaching Bowman's space. Scale bar (C, D) 50 μm.
Figure 3. Three-day incubation of scaffold showing antegrade seeding of vasculature and retrograde seeding of collecting system. H&E (A). GFP+ cells line peritubular capillary vessels (B–D) while a GFP− βTC-tet cell population (D) occupy tubular structures. GFP+ cells are marked by arrows and the GFP− βTC-tet cells with arrowheads (B–D). Scale bar 100 μm.
Figure 4. 3-day incubation of scaffold after retrograde seeding of pancreatic β cells. Cells showing positive insulin immunoreactivity localized to the collecting tubules (A, B) and glomeruli (C, D). Scale bar 200 μm (A–C) 100 μm (D).
Figure 5. (A) Collagen IV immunohistochemical staining of glomerular tuft containing EOMA cells. (B–D) CD31 immunohistochemical staining of EOMA cells lining blood vessels. Scale bar = 50 μM.
