eRNA binding produces tailored CBP activity profiles to regulate gene expression

Figures & data

Figure 1. RNA binding can generate localized CBP activity profiles and tailored patterns of histone acetylation. (A) Locus specificity for RNA binding to CBP. CBP bound RNAs (Orange) correspond to sites of CBP recruitment to chromatin (Blue) and nascent RNA transcription (Gray) at enhancers and promoters; (B) RNA binding to the activation loop of CBP stimulates catalytic HAT activity by displacing the loop from the active site, and thereby promotes CBP dependent histone acetylation (H3K27ac); (C) eRNA binding could generate enhancer specific patterns of CBP activity by differential regulation of CBP activity. At more active enhancers, CBP activity is stimulated more strongly by eRNA binding (green arrows) than at enhancers with weak stimulation of CBP activity (red cross); (D) Differences in the ability of sense and antisense eRNA transcripts to stimulate CBP activity. The eRNA strand that binds and stimulates CBP strongly (Green arrows) could promote associated gene expression more strongly than weaker binding eRNA strands (Red cross).