Bovine leukemia virus pre-miRNA genes’ polymorphism

Figures & data

Figure 1. The schematic map of the proviral BLV DNA (GeneBank: KT122858.1) and locations of BLV-MIRS-F and BLV-MIRS-B oligonucleotides. Genes encoding proteins are shown in green, and genes encoding RNAs are shown in blue. Thick lines indicate exons, and thin lines represent introns.

Table 1. (a). Nucleotide sequences of BLV pre-miR-B alleles. (b). Nucleotide sequences of pre-miR-B alleles and their frequencies of the occurrence in each subgroup (I-st and II-nd) of BLV-positive cows. The significance level of P values is indicated by color: purple – not significant (P pre-miR-B1 = 0.21), pink – highly significant (Ppre-miR-B2 = 0.15 × 10⁻¹², Ppre-miR-B3 = 0.50 × 10⁻¹⁰, Ppre-miR-B4 = 0.59 × 10⁻¹⁵, Ppre-miR-B5 = 0.78 × 10⁻⁹). Pre-miR-B alleles highlighted in green have been considered as contributed to a decrease of WBC counts. Pre-miR-B alleles do not highlight have been considered as contributed to increasing of WBC counts. Pre-miR-B alleles identified in both subgroups have been highlighted in yellow. Loop sequences of pre-miRs-B have been underlined.

Table 2. Unpaired t-test results for groups and subgroups of cows.

P ≤ 0.0001;	II-nd BLV+ (N = 8);	I-st BLV+ (N = 8);
The difference have been considered to be extremely statistically significant.	The WBC reference interval counted from 12,791 to 31,128 WBCs/µL	The WBC reference interval counted from 7,948 to 16,196 WBCs/µL
BLV+ (N = 16);	P = 0.0567;	P = 0.0394;
The WBC reference interval counted from 4,710 to 29,322 WBCs/µL	The difference have been considered to be not quite statistically significant.	The difference have been considered to be statistically significant.
BLV- (N = 26);	P < 0.0001;	P = 0.4359;
The WBC reference interval counted from 5,906 to 16,609 WBCs/µL	The difference have been considered to be extremely statistically significant.	The difference have been considered to be not statistically significant.

Figure 2. Two-way ANOVA test results for BLV-negative, BLV-, (N = 26) and all BLV-positive, BLV+, (N = 16) groups of cows, and for BLV-positive, I-st BLV+, (N = 8) and II-nd BLV+ (N = 8) subgroups of cows. The number of WBCs (y ˣ 10³cells/µL) is on the y-axis.