Figures & data
Figure 1. Schematic of natural and synthetic tRNALeu variants.
![Figure 1. Schematic of natural and synthetic tRNALeu variants.](/cms/asset/dfa45dcc-fa4c-4980-9119-160576ec798d/krnb_a_2340297_f0001_oc.jpg)
Table 1. Naturally occurring human tRNALeu variants characterized in this study.
Figure 2. Identification of Leu mis-incorporation in mammalian cells by tandem mass spectrometry.
![Figure 2. Identification of Leu mis-incorporation in mammalian cells by tandem mass spectrometry.](/cms/asset/ca921449-5654-4e36-aa7e-cff4f7f6a69b/krnb_a_2340297_f0002_oc.jpg)
Table 2. Summary of Leu mis-incorporation identified by MS/MS in mammalian cells.
Figure 3. OTTR-seq analysis of N2a cells expressing wild-type human tRNA or anticodon variants.
![Figure 3. OTTR-seq analysis of N2a cells expressing wild-type human tRNA or anticodon variants.](/cms/asset/9ac9c0b0-c9e0-4154-8c6e-2bc465259cf6/krnb_a_2340297_f0003_oc.jpg)
Figure 4. Global protein synthesis levels in cells expressing tRNA variants.
![Figure 4. Global protein synthesis levels in cells expressing tRNA variants.](/cms/asset/ebcbdb05-39a5-46bc-8104-a2ac9280c58a/krnb_a_2340297_f0004_oc.jpg)
Figure 5. eGFP fluorescence and cytotoxicity in normal and mistranslating cells under proteasome inhibition.
![Figure 5. eGFP fluorescence and cytotoxicity in normal and mistranslating cells under proteasome inhibition.](/cms/asset/a7f38d33-0a63-4df7-9bdf-53f07add7d15/krnb_a_2340297_f0005_oc.jpg)
Figure 6. Impact of tRNALeu anticodon variants on yeast growth.
![Figure 6. Impact of tRNALeu anticodon variants on yeast growth.](/cms/asset/552845d2-fdfe-4bd2-a3c0-6bca59c92b3b/krnb_a_2340297_f0006_oc.jpg)
Figure 7. Identification of Leu mis-incorporation in yeast by tandem mass spectrometry.
![Figure 7. Identification of Leu mis-incorporation in yeast by tandem mass spectrometry.](/cms/asset/22fae63a-b1de-45a0-8dbf-b9fdaa6ac19e/krnb_a_2340297_f0007_oc.jpg)
Figure 8. Relative growth of yeast strains with tRNALeu anticodon variants.
![Figure 8. Relative growth of yeast strains with tRNALeu anticodon variants.](/cms/asset/a57350e3-9813-4f8e-ae22-7da3fa63ba5d/krnb_a_2340297_f0008_oc.jpg)
Figure 9. Analysis of anticodon variants in different contexts.
![Figure 9. Analysis of anticodon variants in different contexts.](/cms/asset/fbae6350-c609-4653-b403-5ccf13b8cb9d/krnb_a_2340297_f0009_oc.jpg)
DataFile1.csv
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Download MS Word (14.4 MB)Data availability statement
All data are available in the figures, tables, supplementary information, and Supplementary Data Files 1–4.
All MS/MS data files are available:
https://figshare.com/articles/dataset/Mass_spec_tRNALeu_2023_zip/23284907/1
https://bioinfor.sharefile.com/d-sb232430cb59d423c99b1cb5e3b492e58
OTTR-seq data files are available at NCBI BioProject database
http://www.ncbi.nlm.nih.gov/bioproject/1066329
SubmissionID:SUB14155213
BioProject ID: PRJNA1066329