Trichloroethylene Does Not Accelerate Autoimmune Diabetes in NOD Mice

Figures & data

FIG 1. Lymphocyte subset analysis (absolute cell count in 10³ cells/ml) in peripheral blood samples from TCE-treated and control mice: total white blood cell (A), lymphocyte (B), B (C), T (D), CD4⁺ T (E), CD8⁺ T (F) cell counts (10³ cells/mL). Presented values are mean ± SEM. *p < 0.05; **p < 0.01.

TABLE 1 Expression of markers of CD4⁺ T-cell activation (CD44, CD54 and CD45RB) on peripheral blood T-cells or splenocytes from female NOD mice (fluorescence intensity)

				Fluorescence intensity
		Type of sample	Age (weeks)	Control	Trichloroethylene
Cell subsets
CD45RB	High	Spleen	15	1255.7±20.4	1180.0±21.8
	Low	Spleen	19	52.5±2.9	72.4±6.5**
	Low	Blood	23	199.6±9.8	255.3±22.0
CD4^+T-cells
CD44	High	Spleen	19	1163.7±23.7	1085.3±25.9
CD54 (ICAM-1)		Spleen	19	336.1±10.0	296.2±5.2**
		Blood	23	300.4±18.7	251.2±9.2

Mean fluorescence intensity from blood or splenic cell suspension of treated mice compared with mean fluorescence intensity from blood or splenic cell suspension of control mice (mean±SEM)

*p < 0.05

**p < 0.01

TABLE 2 Expression of markers of CD8⁺ T-cell activation (CD44, CD54 and CD45RB) on peripheral blood T-cells or splenocytes from female MOD mice (fluorescence intensity)

			Fluorescence intensity
	Type of sample	Age (weeks)	Control	Trichloroethylene
Cell subsets
CD45RB	Spleen	19	1297.9±85.5	1537.5±29.1
CD8^+ T-Cells
CD44	Spleen	23	690.3±19.9	885.6±40.1***
CD54 (ICAM-1)	Blood	23	417.7±29.5	318.82±7.5

Mean fluorescence intensity from blood or splenic cell suspension of treated mice compared with mean fluorescence intensity from blood or splenic cell suspension of control mice (mean ± SEM)

*p < 0.05

***p < 0.001

FIG 2. Progression of the incidence of TCE-treated NOD mice with a high serum level of IFN-gamma and TNF-alpha compared with in control NOD mice from (concentration > 20 pg/ml).

Incidence of histopathological changes in the pancreas, thymus, mesenteric and axillary lymph nodes of TCE-treated and control female NOD mice

	Age (week)
	15		19		23
	Control (n=10)	TCE (n=10)	Control (n=10)	TCE (n=10)	Control (n=10)	TCE (n=7)
Pancréas
Absence of islets	2	1	1	1	3	1
Numerous islets	2	4	2	2	3	4
Lymphoid infiltrate	1	1	5	5	4	2
Thymus
Lymphoid hyperplasia	4	0	5	8	2	0
Mesenteric
Paracorticale hyperplasia	5	4	8	8	10	7
Lymph nodes
Decrease in secondary follicules	4	5	8	9	10	7
Axillary
Paracorticale hyperplasia	4	3	5	8	9	6
Lymph nodes
Decrease in secondary follicules	5	6	7	7	10	6

TABLE 4 Reported role of the markers and effects of trichloroethylene administration on the spontaneous evolution of the autoimmune diseases in MRL^+/+ and NOD mice

Markers	Role in lupus-like disease in MRL^+/+ mice	Trichloroethylene regulation in MRL^+/+ mice	Role in autoimmune diabetes in NOD mice	Trichloroethylene regulation in NOD mice
IFN-γ	Pathogenic	Up	Pathogenic	Down
CD45RB^low CD4^+ T-cells	Pathogenic	Up	Protective	Up
CD45RB^high CD4^+ T-cells	Protective	Down	Pathogenic	Down
CD44 CD4^+ T-cells	Pathogenic	Up	Pathogenic	Down
CD54 CD4^+ T-cells	Pathogenic	Up	Pathogenic	Down