Figures & data
FIG. 4 Effect of immunosuppressive agents on IgM antibody response to SRBC. Rats were treated orally with cyclophosphamide, cyclosporine, prednisolone, or methotrexate for 14 consecutive days. Serum samples were collected at Day 15. The vertical axis shows the serum IgM titer required to yield a value of OD = 1.0. Each bar represents the mean ± SE of 10 animals. Asterisks indicate the value is significantly different from SRBC-immunized vehicle control group (*p < 0.05; ** p < 0.01).
FIG. 1 Change in the number of cells attached to the plate during ELISA. Each bar indicates the mean ± SD.
FIG. 2 Titration curve of anti-SRBC antibodies. IgM (A) and IgG (B) in rats and IgM (C) in mice. Negative control sera were showed open symbols and positive control sera were showed closed symbols. For positive control, pooled anti-sera for IgM and IgG were collected at Days 6 and 14 after immunization (rats: 3× 108 cells, intravenously. and mice: 5 × 108 cells, intraperitoneally). IgM and IgG antibody levels were determined using the whole SRBC-ELISA.
FIG. 3 Potential effect of lot differences in SRBC used for ELISA on IgM antibody response to SRBC. The sera (n = 15/group) were collected at Day 6 after immunization with 3× 108 SRBC of Lot X. IgM antibody levels were determined by whole SRBC-ELISA of Lots X, A, B, and C, respectively. The bar indicates average in each group.
