Comparison of Primary Immune Responses to SRBC and KLH in Rodents

Figures & data

FIG. 1 Left panel: B₆C₃F₁ female mice were sensitized intravenously (IV) with SRBC and evaluated on Day 4 in the Plaque Assay. Data are presented as specific activity (IgM AFC/10⁶ spleen cells) or total spleen activity (IgM AFC/spleen). Middle panel: Animals were sensitized IV with 100 μ g/animal of KLH and serum collected on Day 5. Serum levels of IgM anti-KLH antibody were determined by ELISA. Right panel: Animals were sensitized IV with 2 mg/animal of KLH and serum collected on Day 5. Serum levels of IgM anti-KLH antibody were determined by ELISA. Results are expressed as percent of vehicle control. *p ≤ 0.05 or **p ≤ 0.01 vs. vehicle treated animals. Each group consisted of eight animals.

FIG. 2 Left panel: Sprague–Dawley (SD) female rats were sensitized with SRBC and evaluated on Day 4 in the Plaque Assay. Data are presented as specific activity (IgM AFC/10⁶ spleen cells). Middle panel: Animals were sensitized IV with 100 μ g/animal of KLH and serum collected on Day 6. Serum levels of IgM anti-KLH antibody were determined by ELISA. Right panel: Animals were sensitized IV with 2 mg/animal of KLH and serum collected on Day 6. Serum levels of IgM anti-KLH antibody were determined by ELISA. Results are expressed as percent of vehicle control. *p ≤ 0.05 or **p ≤ 0.01 vs. vehicle-treated animals. Each group consisted of 7–8 animals.