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Abstract
Synthesis of nanoparticles with interesting physicochemical properties using efficient as well as eco-friendly technology is one of the main objectives of nanotechnology. In this work, gold nanoparticles were produced in a bioreactor by E. coli. This research for the first time was carried out at a scale larger than laboratory scale indicating ability to produce gold nanoparticles at a large scale using microorganisms. In addition, Uv–vis spectroscopy, scanning electron microscopy, and dynamic light scattering were employed to investigate characterization of nanoparticles.