Effects of Boron and Salinity on Red Raspberry in Vitro

Figures & data

TABLE 1. Effect of B and NaCl concentration of the culture medium on the length, fresh and dry weights, and fresh-to–dry weight ratio per explant (Rubus idaeus L. ‘Autumn Bliss’)

B (mM)	NaCl (mM)	Shoot length (mm)	Fresh weight (g)	Dry weight (g)	Fresh/dry weight
0.1	0	35.4a	0.92a	0.15a	6.2a
	5	35.1a	0.91a	0.15a	6.3a
	10	34.6a	0.90a	0.14a	6.4a
0.5	0	34.8a	0.90a	0.14a	6.4a
	5	31.5a	0.87ab	0.13b	6.6ab
	10	25.0b	0.80b	0.11b	7.4b
Mean separation within columns followed by different letters are significantly different according to Duncan's multiple range test (P ≤ 0.05).

TABLE 2. Effect of B and NaCl concentration of the culture medium on the total chlorophyll (a + b) and chlorophyll fluorescence of raspberry explants (Rubus idaeus L. ‘Autumn Bliss’)

B (mM)	NaCl (mM)	Total chlorophyll (a + b) (mg g^−1 f.w.)	Fluorescence (Fv/Fm) ^z
0.1	0	2.29a	0.75a
	5	2.11ab	0.73ab
	10	2.07ab	0.72ab
0.5	0	2.12ab	0.73ab
	5	1.95b	0.71b
	10	1.60c	0.68c
^zFv/Fm is the variable fluorescence over the maximum fluorescence.
Mean separation within columns followed by different letters are significantly different according to Duncan's multiple range test (P ≤ 0.05)

TABLE 3. Effect of B and NaCl concentration of the culture medium on B, Cl, and Na concentrations of raspberry explants (Rubus idaeus L. ‘Autumn Bliss’)

B (mM)	NaCl (mM)	B (μg g^−1 d.w.)	Cl (% d.w.)	Na (% d.w.)
0.1	0	87b	0.99c	0.10d
	5	76b	1.03c	0.10d
	10	82b	1.56b	0.37c
0.5	0	210a	1.49b	0.40c
	5	211a	2.13a	0.65b
	10	195a	2.13a	0.69b
Mean separation within columns followed by different letters are significantly different according to Duncan's multiple range test (P ≤ 0.05).

FIGURE 1. The effect of B and NaCl concentration of the culture medium on the a) relative growth rate and b) relative performance of raspberry grown in vitro (Rubus idaeus L. ‘Autumn Bliss’). Bars represent ± SE values.