Figures & data
FIGURE 1 Regeneration of transgenic pineapple plants and their molecular analysis. A. Direct regeneration of shoots from transformed leaf bases; B. Hardened transgenic plants growing in the greenhouse; C., D. Fruiting of transgenic plant in the greenhouse; E. PCR-Southern analysis of eleven transgenic plants: Lanes 4–14, transformed plants (T1–T11), lane 1 marker (M), lanes 2 and 3 +vector and −vector control (C+ and C−), lane 15 blank. Note the hybridization of the probe with 180bp band corresponding to MSI–99 gene. Plants T1 to T5 and T8 to T11–transformed; T6 and T7–untransformed; F. RT-PCR analysis of eleven hardened transformed plants. Note the 180bp band corresponding to MSI–99 in T4 and T10 plants and positive control.
