The Effects of Heat Treatment on the Gene Expression of Several Heat Shock Protein Genes in Two Cultivars of Strawberry

Figures & data

Table 1. Primer pairs designed for qPCR amplification of gene-coding regions of several heat shock protein and heat shock factors in strawberry.^z

Gene annotation^y	Gene ID^y	Primer sequence
Hsp90	01495	F5′-CTGGATGAGGAAGCCAGAAG-3′
		R5′-AAAGGTGCTCTTCCCAGTCA-3′
Hsp70	29595	F5′-CTTCCGTCGCTTACACT-3′
		R5′-AGAAGGTGTTCTCCGGGTTC-3′
Hsp70	26629	F5′-ATTGCTGCCCTTAATGTGCT-3′
		R5′-CCAACACTGGTTGCCTTCTT-3′
HsfA-4a	23802	F5′-ATGGGAATTTGCGAATGATG-3′
		R5′-CAGGTTCGGCAAAGAATGAC-3′
HsfA-6b	20347	F5′-CAAGCTGTAGGCCCTTGTGT-3′
		R5′-CCATCATCAGAACCTGCTTG-3′
sHsp25.1	12739	F5′-TGTCACCAATGCGGACTATG-3′
		R5′-CACCTCCTGATCGGCTTCTT-3′
sHsp24.5	08959	F5′-CAGGGCACTCTGACTGTGAA-3′
		R5′-GAGAACCTCCTCCCTCCATC-3′
sHsp17.4	15418	F5′-TCTGGATGCTCCAATCTTGAC-3′
		R5′-TTGAAGGTCTTCTCGGCATC-3′
sHsp16	11408	F5′-GACTGCCGGGAAATCAGTAG-3′
		R5′-CCATTCTCCACCTGAGCTTT-3′
sHsp15.96	07764	F5′-CTCCCTGAGAATGCCAAGAC-3′
		R5′-GGTGTCCTAGCCTCCACCTT-3′
UBQ	09659	F5′-GACCATTACCCTGGAGGTTGAGAG-3′ F5′-CCCACCACGAAGCCTGAGC-3′
DBP^x	08438	F5′-TTGGCAGCGGGACTTTACC-3′ R5′-CGGTTGTGTGACGCTGTCAT-3′

^zPrimer pairs for the two reference genes are listed. F and R represent the forward and reverse primers, respectively.

^yGene annotations and IDs were obtained from Fragaria vesca Whole Genome v1.1 Assembly and Annotation at the GDR (https://www.rosaceae.org/species/fragaria/fragaria_vesca/genome_v1.1).

^xThe primer sequences of DBP were retrieved from the study of Mehli et al. (2004).

Table 2. Quantitative data for the eight HSP and two HSF genes investigated showing the LSMEANS of gene expression ratio ± standard error for each cultivar normalized with two reference genes: ubiquitin (UBQ) and DNA binding protein (DBP).^z

Gene annotation^y	Gene ID^y	Cultivar	Expression ratio ± SE	p-Value
Hsp90	01495	Festival	32.16 ± 16.25	0.0215
		Ventana	7.51 ± 3.78
Hsp70	26629	Festival	95.19 ± 42.35	0.0363
		Ventana	26.61 ± 11.14
Hsp70	29595	Festival	64.40 ± 21.43	0.2327
		Ventana	120.71 ± 45.94
HsfA4a	23802	Festival	2.32 ± 0.55	0.5439
		Ventana	1.87 ± 0.44
HsfA6b	20347	Festival	3.56 ± 0.87	0.0117
		Ventana	1.13 ± 0.28
sHsp15.96	07764	Festival	9.26 ± 1.89	0.0004
		Ventana	22.98 ± 4.68
sHsp16	11408	Festival	123.80 ± 61.52	0.0366
		Ventana	47.61 ± 23.85
sHsp17.4	15418	Festival	153.55 ± 75.05	0.0085
		Ventana	10.72 ± 5.24
sHsp24.5	8959	Festival	29.48 ± 7.94	0.2089
		Ventana	20.15 ± 5.45
sHsp25.1	12739	Festival	264.74 ± 140.27	0.0209
		Ventana	83.38 ± 43.50

^zThe observed differences in the gene expression ratios between cultivars was determined to be statistically significant for p-values < 0.05.

^yGene annotations and IDs were obtained from Fragaria vesca Whole Genome v1.1 Assembly and Annotation at the GDR (https://www.rosaceae.org/species/fragaria/fragaria_vesca/genome_v1.1).

Mehli, L., J.G. Schaart, T.D. Kjellsen, D.H. Tran, E.M.J. Salentijn, H.J. Schouten, and T.H. Iversen. 2004. A gene encoding a polygalacturonase-inhibiting protein (PGIP) shows developmental regulation and pathogen-induced expression in strawberry. New Phytol. 163:99–110.

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