Efficacy of Passion Fruit Cryopreservation Using Cryopotectant Agents

Figures & data

Table 1. Percentage of germination (PG), germination speed index (GSI), percentage of emergency (PE), emergency speed index (ESI) of Passiflora eichleriana seeds and shoot length (SL), root length (RL), shoot (SDM) and root (RDM) dry mass of P. eichleriana seedlings submitted to different cryoprotectants

Treatments	PG (%)	GSI	PE (%)	ESI	SL (cm)	RL (cm)	SDM (g)	RDM (g)
Without Cryoprotection	56^ns	4.04 a*	91 a*	4.76 a*	6.9 a*	12.3 a*	0.22 a*	0.06 a*
Control	51	3.26 a	83 a	4.34 a	6.1 a	11.1 a	0.22 a	0.08 a
Gly 1.73	54	1.97 b	2 b	0.07 b	0.6 b	2.0 b	0.02 b	0.01 b
Gly 2.28	56	2.40 b	1 b	0.04 b	0.2 b	0.5 b	0.01 b	0.01 b
Gly 2.60	47	1.71 b	4 b	0.17 b	1.0 b	2.9 b	0.04 b	0.02 b
Gly 2.71	51	1.76 b	2 b	0.07 b	0.4 b	1.2 b	0.01 b	0.01 b
Suc 0.37	67	5.29 a	85 a	4.35 a	6.2 a	12.6 a	0.20 a	0.09 a
Suc 0.46	64	4.70 a	90 a	4.91 a	6.4 a	12.4 a	0.22 a	0.08 a
Suc 0.54	64	4.45 a	86 a	4.60 a	6.9 a	12.8 a	0.24 a	0.10 a
Suc 0.61	72	5.14 a	76 a	3.64 a	5.9 a	12.6 a	0.21 a	0.09 a
DMSO 0.37	66.5	3.93 a	82 a	4.25 a	6.7 a	12.1 a	0.24 a	0.10 a
DMSO 0.72	56.0	3.93 a	84 a	4.24 a	6.5 a	11.3 a	0.19 a	0.09 a
DMSO 1.04	37.0	2.28 b	85 a	4.32 a	6.12 a	11.3 a	0.17 a	0.08 a
DMSO 1.35	52.0	2.86 a	82 a	3.96 a	6.0 a	11.2 a	0.18 a	0.09 a
CV %	11.72	10.95	7.82	6.83	6.3	8.3	2.74	1.11

*Means within same column followed by same letter in the column do not differ by Dunnett’s test at the 5% probability level; ^ns not significant. Gly – Glycerol; Suc – Sucrose. Concentrations in mol. Transformed data into arc sin $(X\mathrm{\%}/100{)}^{1/2}$ for percentage data.

Table 2. Percentage of germination (PG), germination speed index (GSI), percentage of emergency (PE), emergency speed index (ESI) of Passiflora cristalina seeds and shoot length (SL), root length (RL), shoot (SDM) and root (RDM) dry mass of P. cristalina seedlings submitted to different cryoprotectants

Treatments	PG (%)	GSI	PE (%)	ESI	SL (cm)	RL (cm)	SDM (g)	RDM (g)
Without Cryoprotection	66 a*	1.73 a*	44 a*	1.07 a*	1.8 a*	5.7 a*	0.07^ns	0.03 a*
Control	60 a	1.49 a	41a	1.01 a	1.7 a	4.5 a	0.07	0.03 a
Gly 1.73	0 b	0.00 b	0 b	0.00 b	0.0 b	0.0 b	0.00	0.00 b
Gly 2.28	8 b	0.21 b	0 b	0.00 b	0.0 b	0.0 b	0.00	0.00 b
Gly 2.60	0 b	0.00 b	0 b	0.00 b	0.0 b	0.0 b	0.00	0.00 b
Gly 2.71	0 b	0.00 b	0 b	0.00 b	0.0 b	0.0 b	0.00	0.00 b
Suc 0.37	57 a	1.41 a	32a	0.81 a	1.6 a	4.8 a	0.08	0.03 a
Suc 0.46	58 a	1.34 a	15 a	0.33 a	0.7 a	2.0 a	0.03	0.01 a
Suc 0.54	57 a	1.46 a	41 a	1.01 a	1.8 a	5.6 a	0.07	0.03 a
Suc 0.61	58 a	1.43 a	25 a	0.62 a	1.4 a	3.9 a	0.21	0.02 a
DMSO 0.37	45 b	0.92 b	38 a	0.94 a	1.9 a	5.2 a	0.08	0.03 a
DMSO 0.72	25 b	0.47 b	44 a	1.11 a	2.0 a	6.2 a	0.087	0.03 a
DMSO 1.04	10 b	0.20 b	46 a	1.12 a	2.0 a	6.9 a	0.09	0.04 a
DMSO 1.35	7 b	0.14 b	2 b	0.05 b	0.6 b	0.8 b	0.05	0.01 b
CV %	13.14	5.89	30.40	14.64	15.8	21.6	6.67	0.96

*Means within same column followed by same letter in the column do not differ by Dunnett’s test at the 5% probability level; ^ns not significant. Gly – Glycerol; Suc – Sucrose. Concentrations in mol. Transformed data into arc sin $(X\mathrm{\%}/100{)}^{1/2}$ for percentage data.

Table 3. Percentage of germination (PG), germination speed index (GSI), percentage of emergency (PE), emergency speed index (ESI) of Passiflora nitida seeds and shoot length (SL), root length (RL), shoot (SDM) and root (RDM) dry mass of P. nitida seedlings submitted to different cryoprotectants

Treatments	PG (%)	GSI	PE (%)	ESI	SL (cm)	RL (cm)	SDM (g)	RDM (g)
Without Cryoprotection	22 a*	0.76 a*	23 a*	0.54 a*	2.6 a*	7.7 a*	0.30 a*	0.10 a*
Controle	29 a	0.93 a	21 a	0.51 a	2.8 a	7.0 a	0.31 a	0.09 a
Gly 1.73	0.5 b	0.01 b	0 b	0.00 b	0.0 b	0.0 b	0.00 b	0.00 b
Gly 2.28	0.5 b	0.01 b	0 b	0.00 b	0.0 b	0.0 b	0.00 b	0.00 b
Gly 2.60	1 b	0.02 b	0 b	0.00 b	0.0 b	0.0 b	0.00 b	0.00 b
Gly 2.71	0 b	0.00 b	0 b	0.00 b	0.0 b	0.0 b	0.00 b	0.00 b
Suc 0.37	23 a	0.77 a	16 a	0.38 a	1.9 a	5.5 a	0.25 a	0.07 a
Suc 0.46	25 a	0.69 a	13 a	0.32 a	1.9 a	4.8 a	0.25 a	0.07 a
Suc 0.54	22 a	0.68 a	11 a	0.26 a	1.6 b	4.4 a	0.21 a	0.06 a
Suc 0.61	26 a	0.73 a	14 a	0.25 a	2.3 a	6.7 a	0.28 a	0.08 a
DMSO 0.37	17.5 b	0.51 b	13 a	0.30 a	1.9 a	4.7 a	0.23 a	0.07 a
DMSO 0.72	9.0 b	0.20 b	1 b	0.03 b	0.3 b	0.4 b	0.02 b	0.01 b
DMSO 1.04	1.0 b	0.02 b	0 b	0.00 b	0.0 b	0.0 b	0.00 b	0.00 b
DMSO 1.35	1.0 b	0.23b	0 b	0.00 b	0.0 b	0.0 b	0.00 b	0.00 b
CV %	19.74	7.72	36.51	8.78	16.77	25.62	5.97	2.30

*Means within same column followed by same letter in the column do not differ by Dunnett’s test at the 5% probability level; ^ns not significant. Gly – Glycerol; Suc – Sucrose. Concentrations in mol. Transformed data into arc sin $(X\mathrm{\%}/100{)}^{1/2}$ for percentage data.