Comparative Analysis of Genetic Diversity in Some Non-commercial Cultivars of Musa L. from Assam, India, Using Morphometric and ISSR Markers

Figures & data

Table 1. List of Musa cultivars with vernacular names, genome groups along with coordinates and altitude of their collection sites

Sl No.	Genotypes/Cultivars	Genome	Latitude	Longitude	Altitude (m)
1	Abor malbhog	AAB	27°11.804ʹ N	94°55.104ʹ E	96
2	Adeel	-	27°11.693ʹ N	94°55.265ʹ E	96
3	Athiya kol	BB	26°42.658ʹ N	89°32.300ʹ E	119
4	Axamiya malbhog	AAB	27°11.693ʹ N	94°55.265ʹ E	96
5	Bangali malbhog	AAB	26°43. 908ʹ N	94°08.304ʹ E	143
6	Baratmoni	AAA	27°11.804ʹ N	94°55.104ʹ E	96
7	Bogi Monohar	ABB	26°43. 908ʹ N	94°08.304ʹ E	143
8	Bokmoni	-	26°43. 908ʹ N	94°08.304ʹ E	143
9	Fessa monohar	ABB	27°28.521ʹ N	94°53.761ʹ E	96
10	Gobin tulashi	AAB	26°08.836ʹ N	91°58.687ʹ E	56
11	Guwahatiya kol	-	27°11.693ʹ N	94°55.265ʹ E	96
12	Jatikol	AAB	27°02.822ʹ N	94°90.986ʹ E	96
13	katiya jahazi	AAA	27°02.822ʹ N	94°90.986ʹ E	96
14	Ximalu monohar	ABB	26°43. 908ʹ N	94°08.304ʹ E	143

Table 2. List of ISSR primers with sequences

Sl. No.	Primers	Primer sequences
1	UBC 812	5′-GAGAGAGAGAGAGAGAA-3
2	UBC 814	5′-CTCTCTCTCTCTCTCTCTA-3′
3	UBC 818	5′-CACACACACACACACAG-3′
4	UBC 836	5′-AGAGAGAGAGAGAGAGYA-3′
5	UBC 840	5′-GAGAGAGAGAGAGAGAYT-3′
6	UBC 843	5′-CTCTCTCTCTCTCTCTCTRA-3′
7	UBC 848	5′-CACACACACACACACARG-3′

Figure 1. Cluster analysis of 14 noncommercial Musa accessions based on the 50 morphological markers

Figure 2. Two-dimensional PCA analysis of bannana accession based on 50 different morphometric markers

[Bt-01 Bharatmoni(AAA), Bt-02 Bokmoni, Bt-03 Jatikol(AAB), Bt-04 Adeel kol, Bt-05 Gobin tulshi(AAB), Bt-06 Assamiya-malbhog(AAB), Bt-07 Guwahatiya kol, Bt-08 Fessa monohar(ABB), Bt-9 Katiya- jahaji(AAA), Bt-10 Abor- monohar(ABB), Bt-11 Athiya kol(BB), Bt-12 Ximolu monohar(ABB), Bt-13 Bangali malbhog(AAB), Bt-14 Bogi monohar(ABB)]

Table 3. List of primers, number of amplified fragments showing percentage of polymorphism generated by ISSR markers

Primers	Total fragments	Polymorphic bands	Polymorphism (%)	Average PIC
UBC-812	8	8	100.00	0.41
UBC-814	9	7	77.78	0.34
UBC-818	9	9	100.00	0.42
UBC-836	8	7	87.50	0.39
UBC-840	10	10	100.00	0.47
UBC-843	11	10	90.91	0.38
UBC-848	7	5	71.43	0.41
Total	62	56	89.66	0.40

Figure 3. ISSR profiles for 14 noncommercial Musa accessions using primer A. UBC-812, B. UBC-814, C. UBC-818, D.UBC-836, E. UBC-840, F.UBC- 843, G.UBC −848; M = Molecular marker, Lanes 1. Bharatmoni(AAA), 2. Bokmoni, 3. Jatikol(AAB), 4. Adeel, 5. Gobin-tulashi(AAB), 6. Assamiya malbhog(AAB), 7. Guwahatiya kol, 8. Fessa-monohar(ABB), 9. Katiya jahaji(AAA), 10. Abor malbhog(AAB), 11. Athiya kol(BB), 12. Ximalu monohar(ABB), 13. Bangali malbhog(AAB), 14. Bogi monohar(ABB)

Table 4. Mean of the ISSR-based pairwise genetic diversity estimates (GDEs) between 14 noncommercial banana cultivars using ISSR primers

	1	2	3	4	5	6	7	8	9	10	11	12	13	14
1	1.00
2	0.54	1.00
3	0.47	0.60	1.00
4	0.44	0.55	0.76	1.00
5	0.69	0.56	0.44	0.37	1.00
6	0.76	0.54	0.47	0.42	0.73	1.00
7	0.37	0.49	0.57	0.63	0.37	0.32	1.00
8	0.40	0.54	0.74	0.72	0.38	0.41	0.69	1.00
9	0.55	0.57	0.46	0.49	0.59	0.64	0.37	0.51	1.00
10	0.43	0.48	0.46	0.43	0.39	0.48	0.35	0.42	0.61	1.00
11	0.28	0.44	0.52	0.61	0.28	0.31	0.66	0.76	0.41	0.35	1.00
12	0.36	0.47	0.59	0.71	0.37	0.37	0.68	0.73	0.44	0.36	0.75	1.00
13	0.47	0.76	0.43	0.40	0.48	0.47	0.44	0.44	0.53	0.47	0.40	0.6	1.00
14	0.40	0.48	0.52	0.64	0.37	0.38	0.64	0.70	0.51	0.45	0.75	0.77	0.47	1.00

Note: Lanes/colums 1 – Bharatmoni(AAA), 2 – Bokmoni, 3 – Jatikol(AAB), 4 – Adeel, 5-Gobin tulashi(AAB), 6 – Assamiya-malbhog(AAB), 7 – Guwahatia kol, 8 – Fessa-monohar(ABB), 9 – Katiya-jahaji(AAA), 10 – Abor-malbhog(AAB), 11 – Athiya-kol(BB), 12 – Ximalu-monohar(ABB), 13 – Bangali-malbhog(AAB), 14 – Bogi-monohar(ABB) (experimental banana are scored and treated as a single entity for clarity. GDEs represent 1 − (minus) Jaccard’s similarity coefficient.

Figure 4. UPGMA cluster analysis of 14 noncommercial Musa accessions based on ISSR data generated by seven primers

Figure 5. 3-D principal component analysis (PCA) of 14 noncommercial bananas showing relationships based on ISSR data