Figures & data
Figure 1. Preparation of pecan explants and their establishment on DKW medium; (a) Explants were taken from the spring flush of mature pecan trees; (b) Shoots were cut to one vegetative bud per node; (c) Explants were cultured individually in jars containing DKW medium
Figure 2. Proliferation of pecan on DKW medium supplemented with varying BA concentrations; (a) Healthy explants were selected from the establishment phase, (b) New shoots from establishment phase were excised for proliferation, (c) Multiple shoots were produced after 21 days of culturing during the proliferation phase
Figure 3. Rooting pecan on DKW media supplemented with IBA; (a) The shoots were induced to root on IBA supplemented MS medium in the dark, (b) Induced shoots were cultured on a mixture of vermiculite and ½ DKW medium, (c) The shoots rooted within 35 days on root development medium
Figure 4. Acclimatization of micropropagated pecan; (a) Rooted plantlets were transferred to a mixture of perlite: peat substrate, (b) Elongation of shoots and growth of new leaves were observed after six weeks, (c) Pecan plantlets were re-potted after three months
Figure 5. Effect of chilling duration (4°C) on the establishment of various pecan cultivars in micropropagation. Means of the columns followed by the same letter are not significantly different according to Duncan’s multiple range test (P ≤ 0.05)
Figure 6. Effect of cutting date of shoots of various pecan cultivars, in days after bud break, on the health of explants (explants without symptom of browning). Means of the columns followed by the same letter are not significantly different according to Duncan’s multiple range test (P ≤ .05)
Figure 7. Effect of soaking duration in 2.5% sodium hypochlorite for disinfestation of various pecan cultivar explants. Means of the columns followed by the same letter are not significantly different according to Duncan’s multiple range test (P ≤ .05)
Figure 8. Effect of different concentrations of BA (µM) on in-vitro shoot proliferation rate of various pecan cultivars. Means of the columns followed by the same letter are not significantly different according to Duncan’s multiple range test (P ≤ .05)
Figure 9. Effect of different IBA concentrations on in vitro rooting of micro shoots originated from various pecan cultivars. Means of columns followed by the same letter are not significantly different according to Duncan’s multiple range test (P ≤ .05)
Table 1. Plants status of different pecan cultivars at acclimatization phase