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Commentary

AIM/LIR-based fluorescent sensors—new tools to monitor mAtg8 functions

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Pages 1074-1078 | Received 20 Nov 2017, Accepted 14 Mar 2018, Published online: 11 May 2018

Figures & data

Figure 1. The AIM/LIR-based fluorescent sensors bind selectively to mAtg8 proteins in an AIM/LIR-dependent manner. Summary of AIM/LIR-based fluorescent sensors constructed by Stolz et al. [Citation27] and Lee et al. [Citation26] is presented in the attached table. Columns present each LC3/GABARAP protein and rows indicate the AIM/LIR-based fluorescent sensor used for detection. Description: efficient sensor binding “+”; most efficient binding and preferential colocalization of a pan-specific sensor “+*”; binding detected in affinity isolation assay “(+)”; improved sensor containing PB1 domain (mCh-PB1-AS3-67) is available “[+]”.

Figure 1. The AIM/LIR-based fluorescent sensors bind selectively to mAtg8 proteins in an AIM/LIR-dependent manner. Summary of AIM/LIR-based fluorescent sensors constructed by Stolz et al. [Citation27] and Lee et al. [Citation26] is presented in the attached table. Columns present each LC3/GABARAP protein and rows indicate the AIM/LIR-based fluorescent sensor used for detection. Description: efficient sensor binding “+”; most efficient binding and preferential colocalization of a pan-specific sensor “+*”; binding detected in affinity isolation assay “(+)”; improved sensor containing PB1 domain (mCh-PB1-AS3-67) is available “[+]”.

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