Ethanol from Sugar Cane Bagasse of Pulp and Paper Mill Effluent by Cryptococcus albidus and Saccharomyces cerevisiae

Abstract

Cryptococcus albidus and Saccharomyces cerevisiae was used for separate hydrolysis and fermentation of sugar cane bagasse for production of ethanol. Lignocellulolytic enzymes, CMCase (34 U/ml), FPase (3 U/ml), β-glucosidase (2.3 U/ml), laccase (32 U/ml), and xylanase (12 U/ml), were assayed by fungus, however, after optimization of process parameters, an increase of 1.5-fold sugar (375 mg/g) from bagasse and production of lignocellulolyic enzymes were determined. The sugar produced by sugar cane bagasse was subsequently treated by Saccharomyces cerevisiae, indicating enhanced production of ethanol at 40 h was 38.4 g/L, reached to a maximum at 50 h, and then it was decreased.

Keywords:

• bagasse
• bioethanol
• decolorization
• delignification
• lignocellulolytic enzymes
• fungus